HA tag peptide is a small affinity tag derived from the hemagglutinin (HA) protein of the influenza A virus. The 9-amino-acid HA tag sequence (YPYDVPDYA) can easily be fused to recombinant proteins, which enables their specific detection, purification, and localization using anti HA tag antibodies. HA epitope tag peptide is known for its wide application in assays such as co-immunoprecipitation, ELISA, and Western blotting to study protein interactions.
HA tag peptide (CAS Nr: 92000-76-5) is synthetized by JPT Peptide Technologies, a leader in custom peptide synthesis for cancer and immunological research. It is fully characterized and optimized for researchers to achieve reproducible results in advanced molecular biology applications.
We also offer biotinylated HA tag peptide variants for enhanced detection and capture applications. The HA tag peptides provided by us are available for research use only, and are not intended for human use!
HA Tag Peptide - YPYDVPDYA - Specifications
- Peptide sequence: H-YPYDVPDYA-OH
- Amount: 5 mg
- Purity: Trial Grade: each peptide purified to > 90% (HPLC/MS)
- Counterion: TFA
- Delivery Format: Freeze-dried in plastic vial
- MW (average): 1102.19
- Application(s): Cancer biomarker research, T-cell immunity
- Condition(s)/Topic(s): Cancer
- Standard Delivery Time: 2-5 days
- CAS: 92000-76-5 (w/o biotin)
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Research areas and applications
HA epitope tag is widely used in molecular and cellular biology to facilitate the detection, purification, and characterization of recombinant proteins. It is broadly applied in cancer research, immunology, recombinant protein studies, and cell engineering, where precise tracking of tagged proteins is required.
Key research areas and applications include:
- Protein Expression and Characterization: HA tag peptide is widely used to verify the expression and integrity of recombinant proteins in various expression systems, including bacteria, yeast, and mammalian cells.
- Protein Detection and Purification: The tag enables efficient detection and affinity purification of recombinant proteins using anti HA tag antibody based applications such as Western blotting, ELISA-based assays, immunoprecipitation (IP), co-immunoprecipitation (Co-IP), and affinity chromatography.
- Protein-Protein Interaction Studies: It is widely used to study protein complexes and identify interacting partners involved in dynamic cellular processes through techniques such as co-immunoprecipitation.
- Subcellular Localization: HA-tagged proteins can be visualized using imaging techniques such as immunofluorescence (IF/ICC) and immunohistochemistry to determine their localization within cellular compartments.
- Live-Cell Imaging Studies: Advanced tools such as HA-specific intracellular antibodies (e.g., ’Frankenbody’) enable real-time tracking of HA-tagged proteins and protein dynamics in living cells and model organisms.
- Drug Discovery and Target Validation: The HA tag supports target validation and drug screening by enabling pull-down assays and analysis of protein interactions with candidate compounds.
- Functional Genomics Research: The HA epitope tag can be integrated into endogenous gene loci using genome editing technologies such as CRISPR, allowing the study of proteins at physiological expression levels.
Advantages of HA Tag Peptide (CAS Nr: 92000-76-5)
- Widely Validated System: The peptide is one of the most established epitope tags, supported by a wide range of high-quality anti HA tag antibodies.
- Minimal Structural Interference: At only 9 amino acids, the HA tag peptide sequence is small (HA tag molecular weight= 1102.19) and generally does not interfere with protein folding, structure, or function.
- High Specificity and Sensitivity: Choosing a high-affinity anti HA tag antibody like 3F10 enable sensitive and specific detection of HA tagged proteins. Techniques such as Western blotting, immunofluorescence (IF/ICC), flow cytometry, and immunoprecipitation can be used.
- Versatile Applications: HA peptide is compatible with a broad range of assays, including ELISA, immunoprecipitation, and protein interaction studies.
- Flexible Tag Placement: The HA-tag can be fused to either the N- or C-terminus of recombinant proteins, allowing flexible experimental design.
- Compatibility with Antibodies: HA tagged proteins can be efficiently purified using anti HA tag antibody-based affinity resins and magnetic beads.
- Mild Elution Conditions: Bound proteins can be eluted using free HA peptide, enabling recovery under gentle conditions that preserve protein structure and interactions.
- Versatility Across Species: The viral ha tag sequence is generally not found in the natural proteomes of bacteria, yeast, or mammalian cells, minimizing cross-reactivity with native proteins.
JPT’s affinity tag peptides
One of the major setbacks in the production of recombinant proteins is the purification of the target protein from other components expressed by the organism. Therefore, recombinant proteins are usually expressed with a short peptide tag that allows for a specified subsequent affinity purification.
JPT Peptide Technologies has substantial, long-standing expertise in providing peptides in all formats, scales and modifications to the global scientific community. All our catalog peptides are provided with HPLC-MS analyses to confirm the identity and demonstrate the high quality of our peptides.
Benefits of JPT’s Peptides- All peptides are made in Germany
- Bulk orders or custom peptide synthesis upon request
- Provision of freeze-dried aliquots for enhanced stability
- Proven track record
- Custom peptide synthesis
Interested in HA tag peptides?
Interested in high-quality tag peptides or need assistance selecting the right product for your research? Our reliable customer support team can support you with product information, custom peptide synthesis, or modified and conjugated peptide designs. We also provide guidance on selecting the right anti HA tag antibody for your application.
Please contact us or reach out to our scientific support team for personalized assistance. We look forward to supporting your research.
