ELISpot Service

What is ELISpot and Why is it Important?

ELISpot (Enzyme-Linked ImmunoSpot) is a highly sensitive technique used to detect and quantify cytokine-producing cells. This method is crucial for a wide range of applications in immunology, allowing researchers to measure the frequency of immune cells that produce a specific cytokine in response to a stimulus. ELISpot Assays are invaluable for advancing research in vaccine development, epitope mapping, and immunomonitoring.

The Power of ELISpot for Immune Response Analysis

JPT Peptide Technologies, the global experts in peptides, are your trusted partner for comprehensive ELISpot and ELISpot Assay services. With over 20 years of expertise in peptide manufacturing and cutting-edge technologies, we are committed to delivering precise, reliable results for your cellular immune response analysis.

Why Choose JPT for Your ELISpot Assay Needs?

Our ELISpot Assay services are backed by state-of-the-art laboratories in Berlin, Germany, operating under the strict guidelines of ISO 9001:2015. This certification ensures that our processes meet the highest standards of quality and consistency. In 2023, we proudly secured the 2nd best score in a global T cell ELISpot proficiency panel, further validating our commitment to excellence.

Applications of ELISpot Assays

ELISpot (enzyme-linked immunosorbent spot) is a highly sensitive method for detecting and quantifying cytokine-producing cells, crucial for various applications:

  • Vaccine Development: Evaluate immunogenicity of different antigens using SpotMix and PepMix.
  • Epitope Discovery & Mapping: Identify specific antigenic regions and epitopes within proteins with EMPS.
  • Immunomonitoring: Screen for responsive individuals post-vaccination or disease with PepMix.
  • Immunogenicity Testing: Detect unwanted immune responses against biologics using PepMix.


Our Comprehensive ELISpot Assay Services

At JPT, we offer a full spectrum of ELISpot Assay services, tailored to meet your specific research needs:

  1. In Silico Peptide Design: Our expert team uses advanced computational tools to design peptides that avoid synthesis and application difficulties, ensuring optimal performance in your assays.
  2. Customized Peptide Specifications: Choose from a range of peptide formats, purities, and modifications, including phospho, biotin, dyes, and more, to suit your unique requirements.
  3. Experiment Design Consultation: Collaborate with our experienced scientists to design experiments that yield the most meaningful and actionable results.
  4. Standardized Experimental Execution: All experiments are performed according to harmonized guidelines and validated SOPs, ensuring consistent, high-quality results.
  5. Professional Data Analysis and Reporting: Receive comprehensive data analysis and reporting, complete with expert interpretation and discussion to help you understand and leverage your findings.

Contact Us for Your ELISpot Assay Needs

Ready to elevate your research with our ELISpot assay services? Contact us today to discuss your project requirements and discover how we can help you achieve your research goals with precision and reliability.


Our PepMix Peptide Pool Solutions
We offer the original PepMixTM. Our catalog features more than 500 different PepMixTM Peptide Pools, the largest variety on the market! And if you need a different purity, amount or antigen we are able to provide you with your custom PepMixTM Peptide Pool.

PepMixTM is the gold standard:
  • We are ISO 9001:2015 certified
  • Each individual peptide is purified and quality controlled 
  • CoA and HPLC-MS data available for each individual batch
  • Capping to avoid false positive T-cell responses by deletion peptides 
  • ADCF policy (animal derived component free)

Epitope Mapping Peptide Sets (EMPS) 
Matrix Pools enable fast identification of epitopes within an antigen while consuming minimal amounts of material. We produce overlapping peptides for an antigen and create matrix pools. Each peptide is present in two matrix pools according to a layout that we design for each antigen. In combination with all the individual peptides in the set you are able to identify and confirm epitopes quickly and efficiently. 

SpotMix Pools are custom small scale peptide pools, purified by dialysis. Therefore, we are able to provide SpotMix Pools very quickly and at low cost. They are optimal for broad antigen target discovery, T-cell epitope discovery and biological screening, and neo-epitope based immune monitoring.
Individual Antigen Peptides

Antigen Peptides

Individual immune dominant epitope peptides for different viruses, bacteria and other species for antigen-specific immune response profiling. 

  • Each peptide is purified to > 90% (HPLC/MS)
  •  Delivery  within 2-5 days
  • HPLC-MS analyses included to confirm the identity and quality
  • ISO 9001:2015 certified

More than 350 Antigen Peptides in stock from more than 40 different species!

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Applications

Applications for ELISpot Service

  • Classic T-cell ELISpot using PBMC for cytokine quantification (e.g. IFN gamma quantification, interleukin quantification)
  • Dual FluoroSpot for simultaneous analysis of two different cytokines 
  • T-cell ELISpot using specific, sorted or depleted cell populations
  • B-cell ELISpot for enumeration of antibody secreting cells

Application Notes


  • "Strategy for Identification of CD8 T-cell Epitopes in a Viral Protein" by Holtappels Full Text
  • "CEFX/EFX ULTRA SuperStim Pools: Superior positive controls for T cell assays with broad MHC-allele and antigen coverage" by Eckey et al. Full Text
  • "A Fast and Low Cost Process for Neo-Epitope Based Immune Monitoring" by Derhovanessian et al . Full Text
  • "Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy" by Lam et al.  Full Text
  • "Cytomegalovirus Protein Spanning PepMix™ Peptide Pools to Discover Changes in T cell immunity in the Aging Population" by Kern  Full Text
  • "T cell Therapy for Viral Infections After Hematopoietic Stem Cell Transplant" by Keirnan et al.  Full Text
  • "Qualification and Use of Peptide Libraries for Clinical Trial Immunomonitoring" by Cox & Hayes Full Text
Benefits

Benefits of ELISpot Service

  • We offer several cell separation techniques to generate and work with your cell population of interest 
  • We use flow cytometry to confirm cell populations after separation
  • We offer additional techniques such as ELISA for further analysis
  • Reliable and robust workflows
  • Strict quality control
  • Competitive pricing
  • ISO 9001:2015 certified quality management system 
References
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Health - made in Germany
Quality Assurance

All production is ISO 9001:2015 certified.

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