ELISpot Service

About ELISpot Assay Service

ELISpot (enzyme-linked immunosorbent spot) is a well-known and highly sensitive method for the analysis of cellular immune responses by the quantification of cytokine-producing cells. 

Main applications are:
  • Vaccine development: testing the immunogenicity of different antigens (SpotMix, PepMix)
  • Epitope discovery & epitope mapping: finding specific antigenic regions and identifying epitopes within proteins respectively (EMPS)
  • Immunomonitoring: screening for responding individuals after vaccination or disease (PepMix)
  • Immunogenicity testing: identification of unwanted immune responses against biologics (PepMix)

Our ELISpot Assay Service

We have been at the forefront of a global T cell ELISpot proficiency panel for the past 7 years and have achieved the 2nd best score in 2023. We offer expertise for your ELISpot project from design to execution! 

Our ELISpot service includes:
  • In silico peptide design to avoid potential synthesis and application difficulties from the outset
  • Selecting the appropriate purity, quantity and specfications to meet your needs
  • Designing the most meaningful experiment in close consultation with the customer 
  •  Performing the experiment according to our standards, which are harmonized and validated by Proficiency Panel guidelines and SOPs
  • Professional data analysis , reporting and discussion of data
Please note: We also offer troubleshooting support and method development services


Peptide Antigens for ELISpot

Our PepMix Peptide Pool Solutions
We offer the original PepMixTM. Our catalog features more than 500 different PepMixTM Peptide Pools, the largest variety on the market! And if you need a different purity, amount or antigen we are able to provide you with your custom PepMixTM Peptide Pool.

PepMixTM is the gold standard:
  • We are ISO 9001:2015 certified
  • Each individual peptide is purified and quality controlled 
  • CoA and HPLC-MS data available for each individual batch
  • Capping to avoid false positive T-cell responses by deletion peptides 
  • ADCF policy (animal derived component free)

Epitope Mapping Peptide Sets (EMPS) 
Matrix Pools enable fast identification of epitopes within an antigen while consuming minimal amounts of material. We produce overlapping peptides for an antigen and create matrix pools. Each peptide is present in two matrix pools according to a layout that we design for each antigen. In combination with all the individual peptides in the set you are able to identify and confirm epitopes quickly and efficiently. 

SpotMix Pools are custom small scale peptide pools, purified by dialysis. Therefore, we are able to provide SpotMix Pools very quickly and at low cost. They are optimal for broad antigen target discovery, T-cell epitope discovery and biological screening, and neo-epitope based immune monitoring.
Individual Antigen Peptides

Antigen Peptides

Individual immune dominant epitope peptides for different viruses, bacteria and other species for antigen-specific immune response profiling. 

  • Each peptide is purified to > 90% (HPLC/MS)
  •  Delivery  within 2-5 days
  • HPLC-MS analyses included to confirm the identity and quality
  • ISO 9001:2015 certified

More than 350 Antigen Peptides in stock from more than 40 different species!

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Applications

Applications for ELISpot Service

  • Classic T-cell ELISpot using PBMC for cytokine quantification (e.g. IFN gamma quantification, interleukin quantification)
  • Dual FluoroSpot for simultaneous analysis of two different cytokines 
  • T-cell ELISpot using specific, sorted or depleted cell populations
  • B-cell ELISpot for enumeration of antibody secreting cells

Application Notes


  • "Strategy for Identification of CD8 T-cell Epitopes in a Viral Protein" by Holtappels Full Text
  • "CEFX/EFX ULTRA SuperStim Pools: Superior positive controls for T cell assays with broad MHC-allele and antigen coverage" by Eckey et al. Full Text
  • "A Fast and Low Cost Process for Neo-Epitope Based Immune Monitoring" by Derhovanessian et al . Full Text
  • "Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy" by Lam et al.  Full Text
  • "Cytomegalovirus Protein Spanning PepMix™ Peptide Pools to Discover Changes in T cell immunity in the Aging Population" by Kern  Full Text
  • "T cell Therapy for Viral Infections After Hematopoietic Stem Cell Transplant" by Keirnan et al.  Full Text
  • "Qualification and Use of Peptide Libraries for Clinical Trial Immunomonitoring" by Cox & Hayes Full Text
Benefits

Benefits of ELISpot Service

  • We offer several cell separation techniques to generate and work with your cell population of interest 
  • We use flow cytometry to confirm cell populations after separation
  • We offer additional techniques such as ELISA for further analysis
  • Reliable and robust workflows
  • Strict quality control
  • Competitive pricing
  • ISO 9001:2015 certified quality management system 
References
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TÜV Rheinland certified
Health - made in Germany
Quality Assurance

All production is ISO 9001:2015 certified.

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