Expert ELISpot Method Development & Troubleshooting Service!
At JPT Peptide Technologies, we have been developing PepMix™ for antigen-specific T-cell stimulation in ELISpot since 2004. In parallel, we have developed expertise in setting up optimal T-cell ELISpot conditions to maximize the use of PepMix™ and ensure accurate and reliable results for your immunology studies. Whether you are involved in vaccine development, epitope mapping, or immunogenicity testing, our comprehensive ELISpot services are designed to support your research every step of the way. That may be helping you develop a robust ELISpot protocol for your application or troubleshooting any problems you may be experiencing with your T-cell ELISpot assays and use of our PepMixes™.
Why Choose JPT for ELISpot Services?
- Recognized Expertise: Our work is featured in the Handbook of ELISPOT (Schnatbaum et al., 2024), demonstrating our leadership in antigen-specific stimulation.
- Proven Performance: In 2023, we achieved the 2nd best score in a global T-cell ELISpot proficiency panel accuracy test among 35 labs. Since 2017, we have been part of this international multiconsortia proficiency panel at the initiative of CIC and CIMT, and are currently still sponsoring tests with our unique CEFX Ultra SuperStim Pool. Its goal is to help researchers and clinicians worldwide evaluate and benchmark their performance in immune monitoring with T-cell ELISpot assays.
- Optimized Protocols: We have developed and implemented harmonized, standardized protocols for reproducible and high-quality data.
- Global Community Involvement: As part of the ELISpot Resource Group, we actively contribute to international workshops and knowledge exchange. Contact us if you are interested in hosting a workshop at your facility!
Take Advantage of our Comprehensive ELISpot Services
- Our customized solutions cover every aspect of ELISpot assay development and execution:
- In Silico Peptide Design: Computationally optimized peptides to avoid synthesis and application challenges.
- Custom Peptide Specifications: Choose from a range of peptide formats, purities, and modifications, including phosphorylation, acetylation, and more, to meet your unique requirements.
- Experimental Design Consultation: Work with our experts to ensure meaningful results.
- Standardized Experiment Execution: All assays follow harmonized guidelines and validated SOPs. Lab performance is continuously monitored annually by an independent proficience panel.
- Professional Data Analysis & Reporting: Receive comprehensive analysis with expert interpretation.
Applications of JPT’s T-Cell ELISpot Service
- ELISpot Method Development – Develop a robust protocol for your application.
- ELISpot Troubleshooting – Analyze and resolve your ELISpot problems.
- Epitope Discovery & Mapping – Identify key antigenic regions.
- Immunomonitoring – Screen for antigen-specific immune responses.
- Immunogenicity Testing – Detect unwanted immune responses to biologics
Our Comprehensive ELISpot Assay Service
At JPT, we offer a full spectrum of ELISpot Assay services, tailored to meet your specific research needs:
- In Silico Peptide Design: Our expert team uses advanced computational tools to design peptides that avoid synthesis and application difficulties, ensuring optimal performance in your assays.
- Customized Peptide Specifications: Choose from a range of peptide formats, purities, and modifications, including phospho, biotin, dyes, and more, to suit your unique requirements.
- Experiment Design Consultation: Collaborate with our experienced scientists to design experiments that yield the most meaningful and actionable results.
- Standardized Experimental Execution: All experiments are performed according to harmonized guidelines and validated SOPs, ensuring consistent, high-quality results.
- Professional Data Analysis and Reporting: Receive comprehensive data analysis and reporting, complete with expert interpretation and discussion to help you understand and leverage your findings.
Ready to Elevate Your Research?
Partner with JPT for reliable, high-quality ELISpot assay solutions. Contact us today to discuss your project and find out how our expertise can advance your research!
What is ELISpot and Why is it Important?
ELISpot (Enzyme-Linked ImmunoSpot) is a highly sensitive technique used to detect and quantify cytokine-producing cells. This method is crucial for a wide range of applications in immunology, allowing researchers to measure the frequency of immune cells that produce a specific cytokine in response to a stimulus. ELISpot Assays are invaluable for advancing research in vaccine development, epitope mapping, and immunomonitoring.
Our PepMix Peptide Pool Solutions
We offer the original PepMixTM. Our catalog features more than 500 different PepMixTM Peptide Pools, the largest variety on the market! And if you need a different purity, amount or antigen we are able to provide you with your custom PepMixTM Peptide Pool.
PepMixTM is the gold standard:
- Each individual peptide is purified and quality controlled
- CoA and HPLC-MS data available for each individual batch
- Capping to avoid false positive T-cell responses by deletion peptides
- ADCF policy (animal derived component free)
Epitope Mapping Peptide Sets (EMPS)
Matrix Pools enable fast identification of epitopes within an antigen while consuming minimal amounts of material. We produce overlapping peptides for an antigen and create matrix pools. Each peptide is present in two matrix pools according to a layout that we design for each antigen. In combination with all the individual peptides in the set you are able to identify and confirm epitopes quickly and efficiently.
SpotMix Pools are custom small scale peptide pools, purified by dialysis. Therefore, we are able to provide SpotMix Pools very quickly and at low cost. They are optimal for broad antigen target discovery, T-cell epitope discovery and biological screening, and neo-epitope based immune monitoring.
Individual Antigen Peptides
Individual immune dominant epitope peptides for different viruses, bacteria and other species for antigen-specific immune response profiling.
- Each peptide is purified to > 90% (HPLC/MS)
- Delivery within 2-5 days
- HPLC-MS analyses included to confirm the identity and quality
More than 350 Antigen Peptides in stock from more than 40 different species!
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