Dabcyl-KTSAVLQSGFRKM-Glu(Edans)-NH2 is an internally quenched FRET peptide substrate for SARS-CoV-2 main protease (Mpro/3CLpro). The peptide contains the viral nsp4/nsp5 cleavage motif KTSAVLQSGFRKM. Cleavage separates the Edans donor from the Dabcyl quencher, increasing fluorescence for real-time measurement of protease activity.
Product Specifications
- Product Code: SP-CAT-091
- Sequence: Dabcyl-KTSAVLQSGFRKM-Glu(Edans)-NH2
- CAS Number: 2642630-08-6
- Molecular Weight: 2080.44 g/mol
- Purity: >95% (HPLC-MS)
- Amount: 1 mg
- Format: Freeze-dried in plastic vial
- Storage: Store at -20°C
- Standard Delivery Time: 2-5 days
Applications
- SARS-CoV-2 Mpro/3CLpro activity assays
- FRET-based protease measurements
- Mpro inhibitor screening
- Enzyme kinetics
Protease Substrates for Viral Protease and Mpro Research
This SARS-CoV-2 Mpro FRET substrate is part of JPT's Protease Substrates range for investigating protease function and inhibition. Peptide substrates with defined cleavage motifs and reporter systems support protease activity measurements, enzyme kinetics and inhibitor screening in viral protease research.
Benefits of the Mpro FRET Substrate and JPT Protease Substrates
The Dabcyl/Edans donor-quencher pair converts Mpro-mediated peptide cleavage into a measurable fluorescence increase, supporting continuous activity and inhibition assays. JPT Protease Substrates combine defined peptide sequences with assay-relevant labels for protease activity and inhibition studies.
Do you need an alternative FRET substrate or viral protease sequence?JPT Custom Peptide Synthesis supports customized cleavage motifs, fluorophores, quenchers and other peptide modifications for protease assay development and screening projects.
