Custom SpikeTides™ Reference Peptides

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SpikeTides™ are the inexpensive and reliable source of custom stable-isotope-labeled peptides (SIL peptides, heavy peptides). SpikeTides™ are also available unlabeled peptides (e.g. proteotypic peptides) for relative protein quantification by mass spectrometry-based assays in targeted proteomics.

Custom reference peptides e.g. proteotypic peptides
For targeted proteomics (protein detection and protein quantification by mass spectrometry)
Inexpensive & fast
Light or stable-isotope-labeled peptides (SIL peptides)
Modifications such as PTMs & cysteine carbamidomethylation
SIL peptides contain a heavy arginine or heavy lysine
For absolute quantified reference peptides please check SpikeTides™ TQL

Also available:

1. SpikeTides™ - unlabeled peptides

SpikeTides™ are customized, inexpensive, proteotypic peptides.

Amount: 30 nmol/peptide
Purity: Unpurified
Length: 9-15 amino acids
C-terminal Arg/Lys
Application: Optimization and validation of multiplexed SRM assays
Minimum Order: 24 peptides
Pooling service available upon request

2. SpikeTides™ L – stable isotope labeled peptides

SpikeTides™_L are stable-isotope-labeled, proteotypic peptides.

Amount: 10 nmol/peptide
Purity: Unpurified
Length: 9-15 amino acids
C-terminal heavy arginine (U-13C6; U-15N4) or lysine (U-13C6; U-15N2)
Application: Development of SRM assays and relative quantification of proteins using a single product
Minimum Order: 24 peptides
Pooling service available upon request

3. Maxi SpikeTides™

Maxi SpikeTides™ are synthesized by standard resin-based custom peptide synthesis instead of high-throughput SPOT synthesis and can therefore be adjusted to your specifications.

Maxi SpikeTides™ enable:

Longer peptides
Larger amounts per peptide
Guaranteed purity for each peptide
No minimum order
Validated pooling service available

Please inquire for your Maxi SpikeTides™ via email!

Applications

Applications for Custom SpikeTides™ Reference Peptides

Development, optimization and validation of mass spectrometry-based proteomics assays
Relative quantification of proteins
Selection of proteotypic peptides with high mass spectrometry signal response
Identification of predominant peptide fragments specific for your proteotypic peptide
Development of kits to quantify entire proteomic pathways
Clinical monitoring of therapeutic intervention and disease status
Mass spectrometry based assays (SISCAPA, MRN, etc.)

Application Note:

SpikeTides™ - Proteotypic Peptides for Large-Scale MS-Based Proteomics
Schnatbaum (2011) Full Text

Testimonials

Testimonials for Custom SpikeTides™ Reference Peptides

"At Cambridge University we have significantly benefited from a suite of SpikeTides™ from JPT which have been developed for use as organelle markers in Arabidopsis. Using these we have developed faster and more accurate methods, using SRM and PRM, for estimating organelle enrichment along linear density gradients. Results have been very instrumental in optimizing downstream methodologies, a process which has delivered improved resolution in ensuing datasets for lower costs than our original methods."
Harriet Parsons, PhD (Department of Biochemistry, University of Cambridge, UK)

"At MSKCC, we have been using JPT for isotopically labeled peptide libraries for developing pathway- and genome-scale atlases for quantitation of cellular signaling. This has been invaluable for the Quantitative Cell Proteomics Atlas (QCPA), a research tool for precise and sensitivity quantitation of cellular signaling, as well as clinical diagnostic assays for medical use."
Alex Kentsis, MD, PhD (Memorial Sloan Kettering Institue, New York, USA)

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