Mass Spectrometry-based Discovery

SpikeTides™ are synthetic proteotypic peptides with C-terminal arginine or lysine, designed to mimic tryptic fragments of proteins. SpikeTides™ peptides can be synthesized as unlabeled and unmodified proteotypic peptides as well as heavy peptides (with stable isotope labeled amino acids), with carbamoylated (alkylated) cysteines and post-translational modifications and, last but not least, as absolutely quantified peptide standards.

Due to the flexibility of our SpikeTides™ platform, SpikeTides™ proteotypic peptides are applicable to many standard assays such as SRM assays (selected reaction monitoring) and MRM assays (multiple reaction monitoring).

Applications for Mass Spectrometry-based Discovery

  • Optimization and validation of SRM assays
  • Relative and absolute quantification of proteins
  • Selection of best proteotypic peptides showing high mass spectrometry signal response
  • Find predominant peptide fragments specific for your proteotypic peptide to be used in MRM transition
  • Develop kits to quantify entire proteomic pathways

Benefits of Mass Spectrometry-based Discovery

  • Thousands of SpikeTides™ at unmatched turnaround times (10 000 peptides per week!)
  • SpikeTides™ absolute quantification approach is more robust and cost-effective than other peptide assays e.g. AAA
  • Fully compatible with downstream analyses like MS and HPLC
  • Chemical barcoding of complex SpikeTides™ mixtures within the JPT quantification tag possible

Selected References

“CHESS: a new Human Gene Catalog Curated from Thousands of Large-scale RNA Sequencing Experiments Reveals Extensive Transcriptional Noise”
Mihaela Pertea et al., Genome Biology (2018) - PMID: 30486838
“Discovery and Quantification of Non-human Proteins in Human Milk”
Jing Zhu et al., J. Proteome Res. (2018) - PMID: 30489082
“Alzheimer’s Disease Phenotype or Inflammatory Insult Does Not Alter Function of L-Type Amino Acid Transporter 1 in Mouse Blood-Brain Barrier and Primary Astrocytes”
Mikko Gynther et al., Pharmaceutical Research (2018) - PMID: 30488131
“Multiplex Assay for Quantification of Acute Phase Proteins and Immunoglobulin A in Dried Blood Spots”
V Vidova et al., J Proteome Res. (2018) - PMID: 30408962
“Multiple-Enzyme-Digestion Strategy Improves Accuracy and Sensitivity of Label- and Standard-Free Absolute Quantification to a Level That Is Achievable by Analysis with Stable Isotope-Labeled Standard Spiking”
JR Wiśniewski et al., Journal of Proteome Research (2018) - PMID: 30336047
“A Rapid Non‐target Screening Method for Determining Prohibited Substances in Urine Using Liquid Chromatography/high‐resolution Tandem Mass Spectrometry”
Han et al., Drug Testing and Analysis (2018) - PMID: 30188018
“Absolute Quantification of Targeted Meat and Allergenic Protein Additive Peptide Markers in Meat Products”
Montowska et al., Food Chemistry (2018) - PMID: n.a.
“Quantification of Protein Markers Monitoring the Pre‐analytical Effect of Blood Storage Time Before Plasma Isolation Using 15N Metabolically Labeled Recombinant”
Ahn et al., Journal of Mass Spectrometry (2018) - PMID: n.a.
“Dysregulation of Mucosal Membrane Transporters and Drug Metabolizing Enzymes in Ulcerative Colitis”
Erdmann et al., Journal of Pharmaceutical Sciences (2018) - PMID: 30267783
“Improving Selectivity and Sensitivity of Protein Quantitation by LC–HR–MS/MS: Determination of Somatropin in Rat Plasma”
Bults et al., Bioanalysis (2018) - PMID: 29952231
“Elucidation of Chemical Modifier Reactivity Towards Peptides and Proteins and the Analysis of Specific fragmentation by MALDI‐CID‐MS2”
Rühl et al., Rapid Communications in Mass Spectrometry (2018) - PMID: 29964304
“Cerebrospinal Fluid C-Reactive Protein in Parkinson's Disease: Associations with Motor and Non-motor Symptoms”
Moghaddam et al., NeuroMolecular Medicine (2018) - PMID: 29980980
“Extracellular Prolyl Oligopeptidase Derived from Activated Microglia is a Potential Neuroprotection Target”
Natunen et al., Basic & Clinical Pharmacology & Toxicology (2018) - PMID: 29998529
“ProteomeTools: Systematic Characterization of 21 Post-translational Protein Modifications by LC-MS/MS
Using Synthetic Peptides”

Zolg et al., Molecular and Cellular Proteomics (2018) - PMID: 29848782
“Analysis of Histone Modifications by Mass Spectrometry”
Moritz Carl Völker‐Albert et al., Current Protocols in Protein Science (2018) - PMID: 18429056

More references

Partner with us

Contact: Tanja Kaan
T: +49-30-6392-7878
X: +49-30-6392-7888

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Quality Assurance

All production is performed according to ISO 9001:2015 standards

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