Mass Spectrometry-based Discovery
SpikeTides™ are synthetic proteotypic peptides with C-terminal arginine or lysine, designed to mimic tryptic fragments of proteins. SpikeTides™ peptides can be synthesized as unlabeled and unmodified proteotypic peptides as well as heavy peptides (with stable isotope labeled amino acids), with carbamoylated (alkylated) cysteines and post-translational modifications and, last but not least, as absolutely quantified peptide standards.
Due to the flexibility of our SpikeTides™ platform, SpikeTides™ proteotypic peptides are applicable to many standard assays such as SRM assays (selected reaction monitoring) and MRM assays (multiple reaction monitoring).
Applications for Mass Spectrometry-based Discovery
- Optimization and validation of SRM assays
- Relative and absolute quantification of proteins
- Selection of best proteotypic peptides showing high mass spectrometry signal response
- Find predominant peptide fragments specific for your proteotypic peptide to be used in MRM transition
- Develop kits to quantify entire proteomic pathways
"SpikeTides™ - Proteotypic Peptides for Large-scale MS-based Proteomics"
by K. schnatbaum
Benefits of Mass Spectrometry-based Discovery
- Thousands of SpikeTides™ at unmatched turnaround times (10 000 peptides per week!)
- SpikeTides™ absolute quantification approach is more robust and cost-effective than other peptide assays e.g. AAA
- Fully compatible with downstream analyses like MS and HPLC
- Chemical barcoding of complex SpikeTides™ mixtures within the JPT quantification tag possible
"Seminal Plasma as a Source of Prostate Cancer Peptide Biomarker Candidates for Detection of Indolent and Advanced Disease"
Neuhaus et al., PLOS ONE (2013) - PMID: 23826311
"Multiple Reaction Monitoring of Multiple Low-Abundance Transcription Factors in Whole Lung Cancer Cell Lysates"
Kim et al., Journal of the Proteome Research (2013) - PMID: 23586733
"Automatic Generation of Predictive Dynamic Models Reveals Nuclear Phosphorylation as the Key Msn2 Control Mechanism"
Sunnåker et al., Sci. Signal. (2013) - PMID: 23716718
"N-Glycoprotein SRMAtlas: A Resource of Mass-spectrometric Assays for N-glycosites Enabling Consistent and Multiplexed Protein Quantification for Clinical Applications"
Hüttenhain et al., Mol Cell Proteomics. (2013) - PMID: 23408683
"Occurrence and Detection of Phosphopeptide Isomers in Large-Scale Phosphoproteomics Experiments"
Courcelles et al., Journal of Proteome Research (2012) - PMID: 22668510
"Using iRT, a Normalized Retention Time for more Targeted Measurement of Peptides"
Reiter et al., PROTEOMICS, Volume 12 (2012) - PMID: 22668510
"Automated Workflow for Large-Scale Selected Reaction Monitoring Experiments"
Malmström et al., Journal of Proteome Research (2012) - PMID: 22283722
"Peptides Quantification by Liquid Chromatography with Matrix-Assisted Laser Desorption/Ionization and Selected Reaction Monitoring Detection"
Lesur et al., Journal of Proteome Research (2012) - PMID: 22897511