CMV pp65 (341-349) - HLA-A*24:02 / HLA-A*23:01 (QYDPVAALF)

The CMV pp65 341-349 peptide (QYDPVAALF) is a defined epitope derived from the immunodominant pp65 protein of human cytomegalovirus (HCMV). In contrast to the more commonly used A*02:01-restricted epitopes, this sequence enables analysis of CMV-specific CD8+ T cell responses in HLA-A*24:02 and HLA-A*23:01 contexts, making it particularly relevant for studies involving diverse donor populations.

The peptide sequence QYDPVAALF is used in T cell assays where alternative HLA restrictions are required to capture CMV-specific immunity beyond the most dominant epitopes. This makes it useful for immune monitoring workflows that aim to avoid bias toward a single HLA background or to expand coverage across cohorts.

The Human Cytomegalovirus (HCMV) 65 kDa phosphoprotein (pp65) Peptide is a high quality epitope peptide for stimulation of antigen-specific T cells in T cell assays such as ELISPOT, ICS, cytotoxicity or proliferation assays. The 65 kDa phosphoprotein (pp65) Peptide, H-QYDPVAALF-OH (Uniprot: P06725 aa: 341-349) from JPT is produced under strict quality control and quality management.

CMV pp65 (341-349) - HLA-A*24:02 / HLA-A*23:01 (QYDPVAALF) - Specifications

• Peptide sequence: H-QYDPVAALF-OH
• Amount: 1 mg
• Purity: Trial Grade: each peptide purified to > 90% (HPLC/MS)
• Delivery Format: Freeze-dried in plastic vial
• Application(s): T-cell immunity
• Condition(s)/Topic(s): Control, Infectious mononucleosis, Opportunistic infections, Cytomegalovirus disease
• Standard Delivery Time: approx. 10 days

Visit our webpage for more information on Peptide Tools to Study CMV

Applications

• ELISPOT and FluoroSpot assays
• Intracellular cytokine staining (ICS)
• Cytotoxic T lymphocyte (CTL) assays
• Immune monitoring across diverse HLA backgrounds
• Expansion of CMV-specific T cell repertoires

Is this peptide right for your research?

This peptide is typically selected when working with HLA-A*24:02 or HLA-A*23:01-restricted systems and when a broader representation of CMV-specific responses is required beyond the most dominant A*02:01 epitopes. It is particularly useful in studies involving diverse donor cohorts or population-level immune profiling.

It is less suitable if your experimental setup is optimized around HLA-A*02:01-restricted readouts or relies on highly immunodominant reference peptides such as NLVPMVATV. In those cases, alternative pp65-derived sequences may provide stronger or more consistent responses.

Your Custom or Scrambled Antigen Peptide!

Do you need a scrambled version of this antigen peptide?
We can produce scrambled versions for all our antigen peptides and other peptide sequences. These scrambled peptide versions are cross-checked against the Uniprot database to ensure that the scrambled sequence does not correspond to any natural sequence. Have a look at Scrambled Antigen Peptides!

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Choose sequence, amount and purity. We will assist you along the way: Custom Peptide Synthesis

JPT’s Antigen Peptides
Find your antigen peptide and select the connected PepMix™ Peptide Pool and MHC Multimer for efficient immune monitoring, mapping of T cell epitopes or development of immunotherapy and vaccines. JPT Peptide Technologies has substantial, long-standing expertise in providing peptides, peptidomimetics, and proteins to the global scientific community. Our highly skilled and committed scientific staff ensures that the most appropriate methods and techniques are selected for every synthesis project. All our catalog peptides are provided with HPLC-MS analyses to confirm the identity and demonstrate the high quality of our peptides.

Benefits of JPT’s Antigen Peptides
- All peptides are made in Germany
- Bulk orders or custom peptide snythesis upon request
- Synthesis protocols designed to avoid toxic contaminants and side products
- Provision of freeze dried aliquots for enhanced stability
- Proven track record for applications in clinical studies
- Order the connected PepMix™ Peptide Pool with your peptide!