Positive Control Pool of 27 peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (e.g. ELISPOT).
CEFT Pool - Premium Grade - Specifications
- Amount: 15 nmol (approx. 25µg) / peptide for stimulation of 2,5 x 108 cells
- Purity: Premium Grade: each peptide purified to >90% (HPLC/MS) - (equivalent to an average purity of 95%)
- Delivery Format: Freeze-dried in glass vial
- Application(s): T-cell Immunity
- Condition(s)/Topic(s): Control, Tetanus, Infectious mononucleosis, Cytomegalovirus disease, Influenza, Flu-like disease
- Standard Delivery Time: 2-5 days
Premium Grade Quality PepMix™
For applications where performance, reproducibility, and stability are critical, our new Premium Grade PepMixes™ feature multiple layers of ongoing quality control:
- Recurrent Performance TestingEach batch is regularly tested via ELISpot assays on two donors. Your Benefits:
- Assay standardization & validation
- Minimized performance variability
- Updated Certificate of analysis (CoA)
- Cytotox TestingEach batch is tested in an in vitro Resazurin-based assay to determine if the dissolved peptides show cytotoxic effects.
- Endotoxin TestingPh. Eur.2.6.14A LAL test or Haemotox rFC test are performed with each batch to detect endotoxins.
- Sterility TestingPh. Eur.2.6.1Each batch is incubated in tryptic soy broth. Later turbidity is determined as indicator of contaminating microorganisms.
- Plus, our trusted quality benchmarks still apply
- > 90% Purity of each peptide in the pool (via HPLC-MS)
- Validated pooling method ensuring the presence of each peptide
- Produced under stringent quality management
- CoA and HPLC-MS data available for each individual peptide batch
- Avoiding false positive T-cell responses by contaminating deletion peptides
- ADCF policy
Customize Your PepMix™ Peptide Pool!
Are you interested in your own custom PepMix™? Choose your sequence, amount, purity and pool format. We will assist you along the way. Custom PepMix™ Peptide Pools.
PepMix™ Peptide PoolsProtein-spanning mixtures of overlapping peptides (PepMix™) are extremely efficient for
- Antigen-specific T cell stimulation in T cell assays (e.g. in ELISpot, ICS, cell-mediated cytotoxicity or proliferation assays)
- In-vitro T cell expansion
- Dendritic cell pulsing
- Immune monitoring
- Cellular immune response profiling
- T cell epitope identification
- Standardization of T cell assays
Benefits of PepMix™
- Each peptide quality controlled and guaranteed for identity and purity
- CoA and HPLC-MS data available
- High batch-to-batch consistency
- No false positive T cell responses by contaminating deletion peptides
- No toxic inhibition of T cell responses due to stringent purification of each peptide
- Minimization of endotoxin contamination due to low bioburden process
- ADCF policy in place
- HLA independent stimulation (with antigen spanning peptide pools)
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.jpg "CEF Pool (standard)")
