Fine-tune T cell Activation with our unique PepMix™ Formats!

With a portfolio of over 500 products, JPT’s PepMix™ Peptide Pool Catalog remains the largest on the market. We offer a wide variety of peptide pools tailored for immune monitoring, cellular immune response profiling, or T cell epitope identification, providing unparalleled resources for cutting-edge research in immunotherapy and vaccine development, immune monitoring and gene therapy. 

So why do we offer all these different peptide pool formats? This week we highlight our PepMix™ peptide pool formats, to explain them and showcase their benefits to help you make the right choice.

Our overlapping PepMix™ Peptide Pool - The Gold Standard: 

Developed and validated by JPT & Charité, Berlin, our standard PepMix™ is defined as a peptide pool consisting of 15mer peptides with an 11mer overlap. This length has been shown to allow sufficient peptide accommodation into the specific MHC complex I and II binding groove conformations. Our PepMixes™ are quality controlled at the individual peptide level and again as a complete pool. 

JPT offers a wide variety of different peptide pool formats that are optimized for various applications. 

• Antigen-specific T cell stimulation in T cell assays (e.g. in ELISpot, cytokine staining, cell-mediated cytotoxicity or proliferation assays) 
• In vitro T cell expansion 
• Dendritic cell pulsing 
• Immune monitoring 
• Cellular immune response profiling 
• T cell epitope identification 
• Standardization of T cell assays 

 Application Notes: 

• ‘CEFX/EFX Ultra SuperStim Pools: Superior positive controls for T-cell assays with broad MHC-allele and antigen coverage'
• 'Cross-reactive T cells enhance immune responses in SARS-CoV-2 infection and vaccination'
• 'T Cell Therapy for Viral Infections after Hematopoietic Stem Cell Transplant' 

References: 

• Effect of XBB.1.5-adapted booster vaccination on the imprinting of SARS-CoV-2 immunity
  Pušnik et al., Vaccines (2024) - PMID: 39572559
  Products used: PepMix™ SARS-CoV-2 (Spike Glycoprotein), PepMix™ SARS-CoV-2 (Spike XBB.1.5) 
• Long-term cellular immunity of vaccines for Zaire Ebola Virus Diseases
  Wiedemann et al., Nature Communications (2024) - PMID: 39227399
  Products used: PepMix™ Zaire Ebola (GP/Mayinga-76) 
• Autoantigen-specific CD4+ T cells acquire an exhausted phenotype and persist in human antigenspecific autoimmune diseases
  Saggau et al., Immunity (2024) - PMID: 39226901
  Product used: PepMix™ Human (MOG) 
• Circulating tumor-associated antigen-specific IFN? + 4-1BB + CD8 + T cells as peripheral biomarkers of treatment outcomes in patients with pancreatic cancer
  Murakami et al., Frontiers in Immunology (2024)
  Products used: PepMix™ Human (CEA), PepMix™ Human (TERT), PepMix™ Human (WT1/WT33), PepMix™ Human (Mucin-1) 

PepMix™ Collections: 

All major protein-spanning PepMixes™ for an organism or cancer type are combined into large convenient collection sets. This allows you to study immune responses to many antigens in parallel. Examples include different viruses such as SARS-CoV-2 variants, EBV, HPV, or HCMV., as well as human antigens from different cancer types, such as melanoma, breast cancer or prostate cancer. 

References: 

• Stem cell memory EBV-specific T-cells control EBV tumor growth and persist in vivo'
  Palianina et al., Science Advances (2024) - PMID: 39178248
  Products used: PepMix™ Collection EBV (25ug/peptide), and other 
• Ineffective control of Epstein-Barr-virus-induced autoimmunity increases the risk for multiple sclerosis
  Vietzen et al., Cell (2023) – PMID: 38091993
  Product used: PepMix™ Collection EBV (2x1ug/peptide) 

PepMix™ Pan Select: 

In contrast to our previously mentioned PepMix™ collections, our PepMix™ Pan Selects are peptide pools that cover only hand-selected immune dominant epitopes from various antigens of a pathogen. This way, we provide the ability to efficiently cover efficiently multiple large antigens from a virus while still eliciting a strong immune response from your sample. 

References: 

• Lymph-node-targeted, mKRAS-specific amphiphile vaccine in pancreatic and colorectal cancer: the phase 1 AMPLIFY-201 trial.
  Pant et al., Nature Medicine (2024) - PMID: 38195752
  Products used: PepMix™ Pan-CMV Select, PepMix™ Pan-EBV Select, and other 
• T Cell Responses against Orthopoxviruses in HIV-Positive Patients
  Stefanie et al., Vaccines (2024)
  Product used: PepMix™ Pan-Poxviridae Select 
• A quest for universal anti-SARS-CoV-2 T cell assay: systematic review, meta-analysis, and experimental validation
  Binayke et al., NPJ Vaccines (2024) - PMID: 38167915
  Products used: PepMix™ Pan-SARS-CoV-2 Select, and other peptide tools 

PepMix™ Ultra Pools: 

Some viruses and cancers have a very high mutation frequency, and therefore come in many sequence variations (e.g. HIV, Influenza etc.). To cover all or many of these sequence variants, we have developed our ULTRA algorithm. PepMix™ ULTRA pools cover sequence diversity within a virus or cancer type using the minimum number of peptides, while maintaining a reliable immune response readout. 

References: 

• Impaired T cells and "memory-like" NK-cell reconstitution is linked to late-onset HCMV reactivation after letermovir cessation Lauruschkat et al., Blood Advances (2024) - PMID: 38315873
  Products used: PepMix™ HIV-1 (NEF) Ultra, and other 
• M.Env ZIKV vaccine protects pregnant rhesus macaques and fetuses against Zika virus infection
  Martinot et al., Research Square (2024)
  Product used: PepMix™ ZIKV (E) Ultra, PepMix™ ZIKV (M) Ultra 
• Cognate Antigen Engagement induces HIV-1 Expression in CD4+ T cells from people in long-term Art
  Moskovljevic et al., BioRxiv (2024)
  Product used: PepMix™ HIV-1 (GAG) Ultra 
• Enhanced detection of antigen-specific T cells by a multiplexed AIM assay
  Lemieux et al., Cell Rep Methods (2024) - PMID: 38228152
  Products used: PepMix™ SARS-CoV-2 (Spike Glycoprotein), PepMix™ HIV-1 (GAG) Ultra, PepMix™ HIV-1 (POL) Ultra, PepMix™ HIV-1 (ENV) Ultra, PepMix™ HIV-1 (NEF) Ultra, and other 

Application Notes: 

• ‘The Challenge of Antigen Sequence Diversity: Solutions with ULTRAPeptide Libraries’ 
• ‘Developing Multi-HIV Antigen Specific T Cells as a Component of a Cure Strategy‘ 

Epitope Mapping Peptide Sets (EMPS): 

These sets contain matrix pools plus individual peptides for a specific protein to provide a rapid and resource-efficient way to identify, pinpoint and confirm epitopes within a protein. Each set includes both matrix pools that cover the entire protein and individual peptides for validation and confirmation, ensuring a streamlined and effective process. 

Custom Options: 

And if you need a different purity, quantity, or antigen, we can provide you with your custom PepMix™ Peptide Pool. Each PepMix™ is quality controlled both at the individual peptide level and as a complete pool. Do you require a fast and cost effective alternative? 

Take a look at our SpotMix Antigen Discovery Pools for antigen target discovery, T cell epitope discovery, and biological screening. Based on our proprietary high-throughput SPOT technology, we synthesize in small quantities, fully automated, and purify via dialysis. SpotMix PLUS Pools come with additional features that make them useful in neo-epitope-based immune monitoring. 

Read up on more references or application notes on our website! 

Contact Us: 

Our team of experts is always here to support your research needs. Contact us today to find out how PepMix™ can advance your immune monitoring projects. Kind Regards, The JPT Team