Expert ELISpot Method Development & Troubleshooting Service!

At JPT Peptide Technologies, we have been developing PepMix™ for antigen-specific T-cell stimulation since 2004. In parallel, we have developed expertise in setting up optimal T-cell ELISpot conditions to maximize the use of PepMix™ and ensure accurate and reliable results for your immunology studies. Whether you are involved in vaccine development, epitope mapping, or immunogenicity testing, our comprehensive ELISpot services are designed to support your research every step of the way. That may be helping you develop a robust ELISpot protocol for your application or troubleshooting any problems you may be experiencing with your T-cell ELISpot assays and use of our PepMixes™. 

Why Choose JPT for ELISpot Services? 

• Recognized Expertise: Our work is featured in the Handbook of ELISPOT (Schnatbaum et al., 2024), demonstrating our leadership in antigen-specific stimulation. 
• Proven Performance: In 2023, we achieved the 2nd best score in a global T-cell ELISpot proficiency panel accuracy test among 35 labs. Since 2017, we have been part of this international multiconsortia proficiency panel at the initiative of CIC and CIMT, and are currently still sponsoring tests with our unique CEFX Ultra SuperStim Pool. Its goal is to help researchers and clinicians worldwide evaluate and benchmark their performance in immune monitoring with T-cell ELISpot assays. 
• Optimized Protocols: We have developed and implemented harmonized, standardized protocols for reproducible and high-quality data. 
• Global Community Involvement: As part of the ELISpot Resource Group, we actively contribute to international workshops and knowledge exchange. Contact us if you are interested in hosting a workshop at your facility!

Take Advantage of our Comprehensive ELISpot Services! 

Our customized solutions cover every aspect of ELISpot assay development and execution: 

In Silico Peptide Design: Computationally optimized peptides to avoid synthesis and application challenges. 

Custom Peptide Specifications: Choose from a range of peptide formats, purities, and modifications, including phosphorylation, acetylation, and more, to meet your unique requirements. 

Experimental Design Consultation: Work with our experts to ensure meaningful results. 

Standardized Experiment Execution: All assays follow harmonized guidelines and validated SOPs. Lab performance is continuously monitored annually by an independent proficiency panel.  

Professional Data Analysis & Reporting: Receive comprehensive analysis with expert interpretation. 

Applications of JPT’s T-Cell ELISpot Service:

• ELISpot Method Development – Develop a robust protocol for your application. 
• ELISpot Troubleshooting – Analyze and resolve your ELISpot problems. 
• Epitope Discovery & Mapping – Identify key antigenic regions. 
• Immunomonitoring – Screen for antigen-specific immune responses. 
• Immunogenicity Testing – Detect unwanted immune responses to biologics.  

Ready to Elevate Your Research? 

Partner with JPT for reliable, high-quality ELISpot assay solutions. Contact us today to discuss your project and find out how our expertise can advance your research!