BioTides are JPT's biotinylated peptides, specifically designed for applications requiring immobilization on streptavidin-coated surfaces such as beads, membranes, glass slides, and microtiter plates.
Manufactured using JPT's proprietary and cost-effective SPOT synthesis technology, each BioTide features an N-terminal biotin linked to the peptide through a flexible hydrophilic spacer. This design improves peptide accessibility after immobilization, helping to maximize target binding and assay performance. In addition, the synthesis strategy enables automatic removal of truncation sequences during immobilization, resulting in screening-quality peptides with performance comparable to purified peptides for many applications.
BioTides can also be customized with non-natural amino acids or post-translational modifications, including phosphorylation and methylation, making them suitable for specialized assay development.
Applications:
BioTides are well suited for a wide range of biomedical screening assays, including:
- Peptide ELISA for epitope and protein-protein interaction mapping
- High-throughput kinase, phosphatase, acetyltransferase, and histone deacetylase substrate screening using platforms such as AlphaScreen®, FlashPlates®, and SPA beads
- Affinity capture and precipitation of interacting proteins
- Peptide microarray production
- Affinity chromatography applications
Check out our peptide catalog with more than 2000 peptides in stock!
Selected References:
- Application Note: BioTides™ as High Throughput Screening Tool for the Identification of Antibody Binding Sites, Kühne et al. (2012)
- Dengue antibody dynamics resolved at epitope level using a dengue 1 human infection model
- Boukaert et al., International Journal of Infectious Diseases (2026)
- Therapeutic treatment of hepatitis E virus infection in pigs with a neutralizing monoclonal antibody
- Hrabal et al., Scientific Reports (2025) - PMID: 40155491
- Affinity maturation and optimization of CD44v6-targeting antibodies for molecular radiotherapy
- Lungren Mortensen et al., Nuclear Medicine and Biology (2025) - PMID: 40253776
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If you would like to learn more or discuss your specific project needs, our team is happy to assist.