Epitope Mapping
Epitope mapping is the process of identifying the specific regions (epitopes) of a protein antigen that are recognized by antibodies or immune receptors such as T cell receptors. Depending on the epitope type, whether linear peptide sequences or conformational (3D) binding sites, different epitope mapping services are available. These include reliable peptide based approaches provided by JPT Peptide Technologies. By identifying immune-relevant regions, these services allow researchers to analyze how antibodies or immune receptors interact with a target, assess the specificity of that interaction, and compare different binding profiles. The resulting information is fundamental for developing immunotherapies, vaccines, and diagnostic assays.
Antibody epitope mapping
Antibody epitope mapping identifies the antigen regions recognized by monoclonal or polyclonal antibodies, providing insight into antibody-target interactions. In a typical peptide-based screening workflow, overlapping antigen-derived peptides spanning the target sequence are screened in peptide array formats by incubating them with the antibody of interest. This enables high-resolution localization of immune-reactive sequences and linear epitopes, while supporting antibody specificity assessment. The resulting epitope information supports therapeutic antibody development, antibody response profiling, and diagnostic research, for example in fields such as neurodegeneration (e.g., Alzheimer’s disease) and autoimmune disorders.
Antibody epitope mapping services by JPT
At JPT Peptide Technologies, we offer high-quality peptide-based epitope mapping services to support your research goals focusing on antibodies. Using synthetic overlapping peptide libraries and high-throughput peptide arrays for screening, our epitope mapping methods provide high-resolution identification of linear epitopes and immune-relevant binding sites across your target protein.
Our services include:
- Overlapping peptide scanning: Sequence-wide screening with overlapping peptides in array formats to localize binding regions across the target protein.
- Linear epitope mapping: High-resolution identification of immune-relevant linear epitopes using overlapping peptide libraries.
- Post-translational modification (PTM) mapping: Detection of immune recognition changes caused by modifications such as phosphorylation and glycosylation.
- Antibody epitope discovery: Discover immune-relevant antibody epitopes to support vaccine development and immune profiling.
Our available peptide arrays (multiwell and high-density PepStar , Peptide ELISA, PepSpots) are depicted in the following figure. They are compatible with antibodies, serum, whole blood, and other biological fluids.
How does our antibody epitope mapping service work?
Send us a short description of your project and we will:
- Provide suggestions on array platform and controls to be used
- Provide a quotation for all-in-one services
- Perform synthesis of peptides and generate peptide microarrays
- Incubate peptide microarrays with your sample
- Evaluate and interpret data
- Assign signals to corresponding peptide sequences
- Quantify signals
- Provide a comprehensive and confidential report
- Provide raw data files
- Provide a functional characterization of mapped epitopes
- Suggest follow-up experiments
T cell epitope mapping
T cell epitope mapping services by JPT
- Overlapping peptide libraries: Sequence-wide coverage of the target antigen for comprehensive T cell epitope discovery.
- Peptide pools for screening: Predefined peptide pool formats to facilitate efficient screening of T cell-reactive regions.
- ELISpot assays: ELISpot assays are also provided as a functional readout option for peptide-induced T cell responses.
General research areas and applications
Our services support a wide range of applications of epitope mapping across different research areas. They are designed for researchers and scientists working in fields such as transplantation studies, autoimmune disorders, cancer immunotherapy, infectious diseases, allergy research, and vaccine development. The main applications in these fields include:
- Antibody development and antibody characterization
- Antibody specificity testing, cross-reactivity assessment, and epitope binning
- Vaccine design and target evaluation
- Antibody and immune response profiling
- T cell epitope identification and cellular immune profiling
- T cell specificity assessment and comparison of antigen-specific responses
- Diagnostic and serological assay development
- Immune escape, variant analysis, and epitope conservation studies
- Mechanism-of-action studies and target engagement analysis
- Biomarker discovery
- Quality control and assay validation for immunological reagents/workflows
Why choose JPT Peptide Technologies?
- Expertise in peptide-based Immunology: Decades of experience in peptide synthesis, peptide microarrays and immunological applications.
- High-throughput screening: Scalable peptide microarray and library-based approaches for rapid discovery with up to 6900 (in triplicates) peptides simultaneously.
- Customizable array design: Tailored mapping strategies to fit your specific research needs.
- Reliable & reproducible data: High-quality synthesis and screening ensuring accurate results.
- High resolution: Allows identification of epitopes at the amino acid level.
- Cost-effectiveness: Using peptide microarrays allows for low cost peptide screening.
- Confidential & expert support: Confidential handling of your project and expert scientific guidance throughout study design, data interpretation, and follow-up planning.
Our related products
FAQ about our epitope mapping services
Why should I choose JPT’s Antibody Mapping Service?
- Hundreds of identical microarray copies can be made from one peptide synthesis
- The immobilized peptides are free of truncated sequences
- Up to 6900 peptides in triplicates per microarray
What kinds of samples does JPT use for its mapping service?
How do I send you my sample?
What do I receive as result in the antibody services?
Looking for guidance?
Have more questions or need support in mapping epitopes? Get in touch with our team to discuss your questions and receive expert guidance for your antibody, T cell, or vaccine related studies.