What is ELISpot & Why is It Important?
ELISpot (Enzyme-Linked ImmunoSpot) is a highly sensitive technique for detecting and quantifying cytokine-producing immune cells. It plays a crucial role in vaccine development, immunogenicity studies, and immune response monitoring by providing precise insights into T-cell activation.
Comprehensive ELISpot Services for T-Cell Immune Monitoring
At JPT Peptide Technologies, we have been pioneering PepMix™-based antigen-specific T-cell stimulation since 2004. Our expertise extends beyond peptide development to optimizing ELISpot assay conditions, ensuring reliable and reproducible results for vaccine research, epitope mapping, and immunogenicity testing.
Why Choose JPT for ELISpot Services?
Expertise – Featured in the Handbook of ELISPOT (Schnatbaum et al., 2024).
Performance – Ranked 2nd in global T-cell ELISpot proficiency testing (2023) among 35 labs.
Protocols – Standardized workflows for consistent, high-quality results.
Global Community – Active member of the ELISpot Resource Group, contributing to international proficiency panels and workshops.
Our ELISpot Services Include
- Custom Peptide Design – Computationally optimized peptides to enhance assay performance.
- Tailored Peptide Specifications – Wide range of formats, purities, and modifications.
- Experiment Consultation – Expert guidance for robust ELISpot assays.
- Standardized Assays– SOP-driven workflows validated by independent proficiency panels.
- Comprehensive Data Analysis – Detailed reports with expert interpretation.
Applications of JPT’s ELISpot Services
- Method Development – Establish optimized ELISpot protocols.
- Troubleshooting – Identify and resolve ELISpot assay issues.
- Epitope Discovery & Mapping – Pinpoint antigenic regions.
- Immunomonitoring – Measure antigen-specific immune responses.
- Immunogenicity Testing – Detect unwanted immune reactions to biologics.
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