Beta-Amyloid (1-40) HFIP treated

Beta-Amyloid (1-40), also known as Abeta (1-40), is a 40-amino-acid peptide derived from the human amyloid precursor protein (APP). It corresponds to the predominant amyloid-β isoform in the brain, presumed to support neuronal function. When its clearance is impaired, it can aggregate into β-sheet-rich oligomers and fibrils, which contribute to amyloid plaque formation and cause neuronal damage. The peptide is therefore closely linked to Alzheimer’s disease and widely used in neurodegenerative research to study amyloid aggregation, plaque formation, and neurotoxicity.

HFIP breaks down pre-formed aggregates and returns beta amyloid (1-40) peptides (CAS: 131438-79-4) to a monomeric state, ensuring a clean, reproducible starting material for experiments. For research use only, do not use in humans!

Produced by JPT Peptide Technologies, a leader in custom peptide synthesis for Alzheimer’s research.

Synthetic peptide derived from the N-terminal region of human Amyloid beta A4 protein (Swiss-Prot ID: P05067). HFIP treatment is performed to disrupt beta-sheets and other unwanted secondary structures.

Beta-Amyloid (1-40) HFIP treated - Specifications

• Purity: >95% (HPLC-MS)
• Delivery Format: Freeze-dried in plastic vial
• CAS: 131438-79-4
• Application(s):
• Condition(s)/Topic(s): Alzheimer's disease
• Standard Delivery Time: 2-5 days

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Role of Beta amyloid (1-40) in Alzheimer’s disease?

Beta amyloid (1-40) is one of the main peptides produced when the amyloid precursor protein (APP) is cleaved by β- and γ-secretases. It is the most abundant soluble form of amyloid beta peptides in the brain and is found at higher levels than Aβ (1-42). Although Amyloid beta 1-40 aggregates more slowly, it plays an important regulatory role in Alzheimer’s disease by interacting with Aβ (1-42), shaping fibril structure, and influencing plaque stability and toxicity.

Impaired production or clearance of Amyloid beta (1-40) also contributes to vascular amyloid deposition (cerebral amyloid angiopathy), making it a key component of the broader amyloid cascade linked to Alzheimer’s pathology.

Visit our webpage to learn more about Alzheimer’s Disease and Amyloid Beta Peptides!

Research areas and applications of Beta Amyloid (1-40):

• Neurodegeneration and Alzheimer’s research: Used to investigate how Beta amyloid (1-40) production, clearance, and aggregation contribute to Alzheimer’s or other neurodegenerative diseases like dementia.
• Amyloid aggregation and plaque formation studies: Serves as a model for studying β-sheet formation and the progression from oligomers to protofibrils and mature fibrils using structural techniques such as NMR, AFM, and cryo-EM.
• Mechanisms of neurotoxicity: Employed to analyze how soluble oligomers disrupt synaptic signaling, induce oxidative stress, trigger apoptosis, and impair neuronal membrane integrity.
• Cerebrovascular research: Used to study the impact of 40-amino-acid beta amyloid isoform on cerebral blood vessels, including vascular dysfunction, impaired blood-flow regulation, and mechanisms underlying cerebral amyloid angiopathy (CAA).
• Neuroinflammation research: Applied in studies exploring microglial activation, cytokine release, and inflammatory responses triggered by aggregated beta amyloid species.
• Biomarker development and diagnostics: Supports the development of CSF and blood biomarkers (e.g., Aβ (1-42)/Aβ (1-40) ratio) that strongly correlate with amyloid PET imaging, providing an indirect link to PET-based Alzheimer’s diagnosis.
• Anti-amyloid drug discovery and therapeutic development: Utilized to screen inhibitors of aggregation, test monoclonal antibodies targeting Aβ peptides, evaluate peptide-based therapeutics, and model the effects of candidate compounds that reduce toxicity or promote clearance.
• Systemic health research: Employed in studies investigating links between circulating Amyloid-beta (1-40) levels and systemic disorders such as kidney dysfunction and cardiovascular diseases.
• Physiological function studies: Used to examine potential normal roles of low-level Beta-amyloid (1-40) in synaptic regulation, neural development, and antioxidant activity.
• Comparison studies with Amyloid beta (1-42): Used in comparison studies with Amyloid beta (1-42) to evaluate differences in concentration, aggregation behavior, and diagnostic value in Alzheimer’s research.

Benefits and limitations:

A brief overview of the strengths and limitations of Abeta (1-40) in experimental studies is shown below:

Key Concepts

What is a HFIP treatment?

Hexafluoroisopropanol (HFIP) is a highly fluorinated organic solvent commonly used in peptide research because it can disrupt hydrogen bonding and dissolve otherwise stable peptide aggregates. In amyloid-beta studies, HFIP treatment breaks down pre-formed fibrils or oligomers by disrupting their β-sheet structures. This process restores amyloid beta peptides to a monomeric state, providing reproducible and well-defined starting material for controlled experiments.

JPT's Single Catalog Peptides
Beta Amyloid 1-40 belongs to our Single Catalog Peptides and is manufactured according to the same high-quality standards applied across our peptide catalog. JPT Peptide Technologies has substantial, long-standing expertise in providing peptides, peptidomimetics, and proteins to the global scientific community. Our highly skilled and committed scientific staff ensures that the most appropriate methods and techniques are selected for every synthesis project. All of JPT's catalog peptides are provided with HPLC-MS analyses to confirm the identity and demonstrate the high quality of our peptides.

Benefits of JPT's Single Catalog Peptides
- Synthesis protocols designed to avoid toxic contaminants and side products
- Provision of freeze dried aliquots for enhanced stability
- Proven track record for applications in clinical studies