AT-MA_01

Acetylome - Deacetylase Microarray

US$547.00

Description

Product Description

Peptide microarray displaying 5599 peptides derived from reported human acetylation sites (plus 384 control peptides). All 5599 peptides carry acetylated lysine residues. Readout is performed by differential measurement, allowing comparative analysis in absence (control) and presence of deacetylase (or other) activities. A control microarray is provided with each order.


Amount: 1 microarray (3 subarrays per slide)
Delivery Format: 1-sample peptide microarray
Application(s): Acetyltransferase assays, Substrate identification
Indication(s)/Topic(s): Acetyltransferase, Deacetylase, Epigenetics
Delivery Time: approx. 3 weeks

Acetyltransferase & Deacetylase Microarrays
JPT Peptide Technologies applies its unique peptide microarray platform to generate peptide microarrays on glass slides for characterization of enzymes such as acetyltransferases, methyltransferases and deacetylases. Peptides are immobilized on glass slides via a flexible linker. Chemoselective coupling generates microarrays displaying covalently attached peptides. Multiple identical copies of each microarray are produced. Incubation can be performed manually or automated. Read-out via fluorescence results in low background and high sensitivity.

Benefits of JPT's Acetyltransferase & Deacetylase Microarrays
- Unique collection of all identified human acetylation sites
- Get hundreds of identical microarray copies
- Peptides are free of truncated sequences
- Small amounts of your precious samples are needed for incubation
- Applicable to a variety of biological samples (e.g. serum, lysate, phosphatase)
- Low background on glass surface. Read-out via fluorescence
- Save time for HTS assay setup
- Screen thousands of potential peptide substrates economically

References

References for Acetylome - Deacetylase Microarray

References:
Read References with Enzyme Substrate Microarrays

Application Notes for Enzyme Substrate Microarrays
Exploring the human acetylome using high-density peptide microarrays
Masch et al., Application Note (2012) (full text)
Unraveling Phosphorylation of the Splicing Factor SC35 by the Dual Signaling Kinase PKC in Human T-Cells
Rao et al., Application Note (2016) (full text)
Use of High-Density Histone Peptide Arrays for Parsing the Specificity of a Histone Modifying Enzyme Complex
Wilczek et al., Application Note (2013) (full text)

Testimonial
"We have successfully applied JPT's peptide microarrays as a screening tool for potential kinases transfering phosphate to phosphorylation sites identified by mass spectrometry. The triplicate alignment as well as the thorough selection of controls enabled generation of high quality data confirming our in vivo data generated by means of affinity chromatographic procedures."
Marcelo P. Coba, PhD, The Wellcome Trust Sanger Institute, Cambridge, UK

Documentation

Documentation for Acetylome - Deacetylase Microarray

Properties

Properties of Acetylome - Deacetylase Microarray

Properties Values
Application: Acetyltransferase assays, Substrate identification
Category: Enzyme Substrate Arrays
Indication / Topic: Acetyltransferase, Deacetylase, Epigenetics
Layout: 1-sample peptide microarray
Organism: Homo sapiens (Human)
Protein Name: Selected proteins
amount: 1 microarray (1" x 3")

Further Information to Acetylome - Deacetylase Microarray

Information Values
Sequence: See Sequence List under Product Documentation
Specifications: 13 mer peptides selected form the human proteome
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Health - made in Germany
Quality Assurance

All production is performed according to ISO 9001:2015 standards.