AT-MA_02
Peptide microarraydisplaying 5599 peptides derived from reported human acetylation sites (plus 384 control peptides). All 5599 peptides carryunmodified lysine residues. Readout is performed by differential measurement, allowing comparative analysis in absence (control) and presence of acetylase (or other) activities. A control microarray is provided with each order.
Are you interested in your custom Peptide Microarrays? Choose your sequences, and array format. We will assist you along the way. Custom PepStar Peptide Microarrays.
Acetyltransferase & Deacetylase Microarrays
JPT Peptide Technologies applies its unique peptide microarray platform to generate peptide microarrays on glass slides for characterization of enzymes such as acetyltransferases, methyltransferases and deacetylases. Peptides are immobilized on glass slides via a flexible linker. Chemoselective coupling generates microarrays displaying covalently attached peptides. Multiple identical copies of each microarray are produced. Incubation can be performed manually or automated. Read-out via fluorescence results in low background and high sensitivity.
Benefits of JPT's Acetyltransferase & Deacetylase Microarrays
- Unique collection of all identified human acetylation sites
- Get hundreds of identical microarray copies
- Peptides are free of truncated sequences
- Small amounts of your precious samples are needed for incubation
- Applicable to a variety of biological samples (e.g. serum, lysate, phosphatase)
- Low background on glass surface. Read-out via fluorescence
- Save time for HTS assay setup
- Screen thousands of potential peptide substrates economically
References:
Read References with Enzyme Substrate Microarrays
Application Notes for Enzyme Substrate Microarrays
Exploring the human acetylome using high-density peptide microarrays
Masch et al., Application Note (2012) (full text)
Unraveling Phosphorylation of the Splicing Factor SC35 by the Dual Signaling Kinase PKC in Human T-Cells
Rao et al., Application Note (2016) (full text)
Use of High-Density Histone Peptide Arrays for Parsing the Specificity of a Histone Modifying Enzyme Complex
Wilczek et al., Application Note (2013) (full text)
Testimonial
"We have successfully applied JPT's peptide microarrays as a screening tool for potential kinases transfering phosphate to phosphorylation sites identified by mass spectrometry. The triplicate alignment as well as the thorough selection of controls enabled generation of high quality data confirming our in vivo data generated by means of affinity chromatographic procedures."
Marcelo P. Coba, PhD, The Wellcome Trust Sanger Institute, Cambridge, UK
| Properties | Values |
|---|---|
| Amount: | 1 microarray (1" x 3") |
| Application: | Enzymology |
| Category: | Enzyme Substrate Arrays |
| Condition / Topic: | Acetyltransferase, Deacetylase, Epigenetics |
| Layout: | 1-sample peptide microarray |
| Organism: | Human |
| Protein Name: | Selected proteins |
| Quantification: | Yes |
| Information | Values |
|---|---|
| Sequence: | See Sequence List under Product Documentation |
| Specifications: | 13 mer peptides selected form the human proteome |
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