Ac-DEVD-pNA is a chromogenic peptide substrate for colorimetric measurement of DEVD-cleaving caspase activity, including caspase-3 and caspase-7. Proteolytic cleavage releases para-nitroaniline (pNA), producing an absorbance signal that can be monitored at approximately 405 nm.
Product Specifications
- Product Code: SP-CAT-089
- Sequence: Ac-DEVD-pNA
- Full Sequence: Ac-Asp-Glu-Val-Asp-pNA
- CAS Number: 189950-66-1
- Molecular Formula: C26H34N6O13
- Molecular Weight: 638.58 g/mol
- Purity: >95% (HPLC-MS)
- Amount: 5 mg
- Format: Freeze-dried in plastic vial
- Storage: Store at -20°C
- Standard Delivery Time: 2-5 days
Applications
- Caspase-3 and caspase-7 activity assays
- Colorimetric protease assays
- Apoptosis research
- Enzyme kinetics
- Caspase inhibitor studies
Chromogenic Protease Substrates for Caspase Assays
Ac-DEVD-pNA belongs to JPT's Protease Substrates portfolio for biochemical analysis of proteolytic enzymes. Synthetic chromogenic and fluorogenic peptide substrates provide practical tools for studying enzyme activity, cleavage preferences and inhibitor effects in protease research.
Benefits of Ac-DEVD-pNA and JPT Protease Substrates
Release of the pNA chromophore provides a straightforward absorbance-based readout for DEVD-cleaving caspase activity, making Ac-DEVD-pNA useful for colorimetric enzyme assays. JPT Protease Substrates provide defined peptide reagents for activity measurements, kinetic studies and inhibitor evaluation.
Do you require another chromogenic substrate or a custom sequence?With JPT Custom Peptide Synthesis, peptide sequences can be produced with project-specific modifications, reporter groups, purity levels and quantities for your assay requirements.
