Ac-DEVD-AMC is a fluorogenic peptide substrate used to measure DEVD-cleaving caspase activity, particularly caspase-3 and caspase-7. The Asp-Glu-Val-Asp (DEVD) recognition sequence is linked to 7-amino-4-methylcoumarin (AMC); proteolytic cleavage releases AMC and enables fluorescence-based detection of enzyme activity.
Product Specifications
- Product Code: SP-CAT-088
- Sequence: Ac-DEVD-AMC
- CAS Number: 169332-61-0
- Molecular Formula: C30H37N5O13
- Molecular Weight: 675.64 g/mol
- Purity: >95% (HPLC-MS)
- Amount: 5 mg
- Format: Freeze-dried in plastic vial
- Storage: Store at -20°C
- Standard Delivery Time: 2-5 days
Applications
- Caspase-3 and caspase-7 activity assays
- Apoptosis research
- Protease activity measurements
- Enzyme kinetics
- Caspase inhibitor studies
Protease Substrates for Caspase Activity Research
Ac-DEVD-AMC is part of JPT's Protease Substrates portfolio of synthetic peptide substrates for studying proteolytic enzyme activity. The portfolio supports research into caspase activity, substrate recognition, enzyme kinetics and protease inhibition in biochemical assays.
Benefits of Ac-DEVD-AMC and JPT Protease Substrates
The DEVD-AMC design combines an established caspase recognition motif with a fluorogenic AMC reporter, providing a convenient readout for quantitative protease activity studies. JPT Protease Substrates offer defined synthetic peptides for enzyme activity, specificity and inhibition assays.
Do you need a different protease substrate or peptide sequence?JPT also provides Custom Peptide Synthesis for projects requiring alternative sequences, reporter groups, modifications, purity levels or project-specific formats.
