Absolute Quantified Peptides -SpikeTides™ TQL

Absolute quantified peptides are used as internal references or peptide standards (absolute quantified reference peptides) for protein quantification (protein quantitation) in mass spectrometry-based proteomics, e.g. for selected reaction monitoring (SRM), multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM).
 
Our proprietary absolute peptide quantification (peptide quantitation) method overcomes the limitations of traditional methods such as limited accuracy and high costs:

  • JPT’s QTag is a small chemical tag attached to the peptide
  • QTag is released by trypsin and does not interfere with measurements
  • Peptide quantification via HPLC-UV or HPLC-MS and UV
  • Robust, reproducible & accurate method (variation CV= 5%)
  • Validated in numerous peer-reviewed papers
  • Lower cost than traditional methods

Also available: 

Comparison of Absolute Quantified Peptides:

  SPIKETIDES™_TQL SPIKETIDES™_TQL PLUS
Quantification by HPLC-UV X X
Quantification by end-user possible X
Amount 5 x 1nmol 5 x 10nmol
Isotopical purity 99% 99%
Incoming material inspection X X
Vendor qualification X X
Batch documentation & CoA X X
Optional: certified vials X
Optional: additional QC methods X


Select your Absolute Quantified Peptides:
1. SpikeTides™_TQ – absolute quantified, unlabeled peptides
SpikeTides™_TQ are quantified, proteotypic peptides with a C-terminal QTag that is cleaved by tryptic digestion.
  • Amount: 5 x 1 nmol/target peptide
  • Purity: Purified
  • Application: Absolute protein quantification
  • Validated pooling service available

2. SpikeTides™_TQL – absolute quantified, SIL peptides
SpikeTides™_TQL are heavy labeled, absolute quantified, proteotypic peptides with a C-terminal QTag that is cleaved by tryptic digestion.
  • Amount: 5 x 1 nmol/target peptide
  • Purity: Purified
  • Application: Absolute protein quantification
  • Validated pooling service available

3. Maxi SpikeTides TQ & TQL
Maxi SpikeTides™ TQ & TQL are synthesized by standard resin-based custom peptide synthesis instead of high-throughput SPOT synthesis and can therefore be adjusted to your specifications.
Maxi SpikeTides™ TQ & TQL enable:
  • Longer peptides
  • Larger amounts per peptide
  • Guaranteed purity for each peptide
  • Classical AAA quantification of peptides
  • Validated pooling service available
Please inquire for your Maxi SpikeTides™ via email!

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Applications

Applications for Absolute Quantified Peptides -SpikeTides™ TQL

  • Achieve accurate and efficient mass spectrometric protein quantitation
  • Find predominant peptide fragments specific for protein biomarkers
  • Detect gene silencing at protein level

Application Notes:
SpikeTides™ for Subcellular Marker Proteins offer Improved Analysis of Complex Plant Samples
Hooper et al. (2017) Full Text
Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides
Bindel et al. (2015) Full Text

Benefits

Benefits of Absolute Quantified Peptides -SpikeTides™ TQL

  • Nondestructive quantification method
  • HPLC-purified peptides
  • Ptoential for monitoring of peptide stability, precipitation and adhesion
  • Absolute and direct quantitation
  • SpikeTides™ TQL approach is robust and cost effective
  • Fully compatible with downstream analyses like MS and HPLC
  • Potential for chemical barcoding of complex SpikeTides™ mixtures within the QTag

Testimonials

Testimonials for Absolute Quantified Peptides -SpikeTides™ TQL

"My unit at the Banting and Best Department of Medical Research, University of Toronto Centre for Cellular and Biomolecular Research (CCBR) works on the systematic identification and quantification of proteins and protein complexes on a global level. In addition we have started to perform targeted proteomics to study protein regulation within pathways, e.g. signaling pathways during stem cell fate decision. For this we used JPT's various SpikeTides™ peptide products to build LC-MRM assays in a fast and cost efficient manner. More recently we also found new ways of using low cost SpikeTides™ as quantitative peptide standards in order to quantify protein targets more accurately. We are currently in the process of validating this technology for the accurate quantification of various predicted microRNA targets."
Prof. Andrew Emili (Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Canada)

Read more Testimonials

References

References for Absolute Quantified Peptides -SpikeTides™ TQL

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Quality Assurance

All production is performed according to ISO 9001:2015 standards.