Absolute Quantified Peptides -SpikeTides™ TQL

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Absolute quantified peptides are used as internal references or peptide standards (absolute quantified reference peptides) for protein quantification (protein quantitation) in mass spectrometry-based proteomics, e.g. for selected reaction monitoring (SRM), multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM).

Our proprietary absolute peptide quantification (peptide quantitation) method overcomes the limitations of traditional methods such as limited accuracy and high costs:

JPT’s QTag is a small chemical tag attached to the peptide
QTag is released by trypsin and does not interfere with measurements
Peptide quantification via HPLC-UV or HPLC-MS and UV
Robust, reproducible & accurate method (variation CV= 5%)
Validated in numerous peer-reviewed papers
Lower cost than traditional methods

Also available:

Comparison of Absolute Quantified Peptides:

	SPIKETIDES™_TQL	SPIKETIDES™_TQL PLUS
Quantification by HPLC-UV	X	X
Quantification by end-user possible		X
Amount	5 x 1nmol	5 x 10nmol
Isotopical purity	99%	99%
Incoming material inspection	X	X
Vendor qualification	X	X
Batch documentation & CoA	X	X
Optional: certified vials		X
Optional: additional QC methods		X

Select your Absolute Quantified Peptides:

1. SpikeTides™_TQ – absolute quantified, unlabeled peptides

SpikeTides™_TQ are quantified, proteotypic peptides with a C-terminal QTag that is cleaved by tryptic digestion.

Amount: 5 x 1 nmol/target peptide
Purity: Purified
Application: Absolute protein quantification
Validated pooling service available

2. SpikeTides™_TQL – absolute quantified, SIL peptides

SpikeTides™_TQL are heavy labeled, absolute quantified, proteotypic peptides with a C-terminal QTag that is cleaved by tryptic digestion.

Amount: 5 x 1 nmol/target peptide
Purity: Purified
Application: Absolute protein quantification
Validated pooling service available

3. Maxi SpikeTides TQ & TQL

Maxi SpikeTides™ TQ & TQL are synthesized by standard resin-based custom peptide synthesis instead of high-throughput SPOT synthesis and can therefore be adjusted to your specifications.

Maxi SpikeTides™ TQ & TQL enable:

Longer peptides
Larger amounts per peptide
Guaranteed purity for each peptide
Classical AAA quantification of peptides
Validated pooling service available

Please inquire for your Maxi SpikeTides™ via email!

Applications

Applications for Absolute Quantified Peptides -SpikeTides™ TQL

Achieve accurate and efficient mass spectrometric protein quantitation
Find predominant peptide fragments specific for protein biomarkers
Detect gene silencing at protein level

Application Notes:

SpikeTides™ for Subcellular Marker Proteins offer Improved Analysis of Complex Plant Samples
Hooper et al. (2017) Full Text

Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides
Bindel et al. (2015) Full Text

Benefits

Benefits of Absolute Quantified Peptides -SpikeTides™ TQL

Nondestructive quantification method
HPLC-purified peptides
Ptoential for monitoring of peptide stability, precipitation and adhesion
Absolute and direct quantitation
SpikeTides™ TQL approach is robust and cost effective
Fully compatible with downstream analyses like MS and HPLC
Potential for chemical barcoding of complex SpikeTides™ mixtures within the QTag

Testimonials

Testimonials for Absolute Quantified Peptides -SpikeTides™ TQL

"My unit at the Banting and Best Department of Medical Research, University of Toronto Centre for Cellular and Biomolecular Research (CCBR) works on the systematic identification and quantification of proteins and protein complexes on a global level. In addition we have started to perform targeted proteomics to study protein regulation within pathways, e.g. signaling pathways during stem cell fate decision. For this we used JPT's various SpikeTides™ peptide products to build LC-MRM assays in a fast and cost efficient manner. More recently we also found new ways of using low cost SpikeTides™ as quantitative peptide standards in order to quantify protein targets more accurately. We are currently in the process of validating this technology for the accurate quantification of various predicted microRNA targets."
Prof. Andrew Emili (Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Canada)

References

Quality Assurance

All production is ISO 9001:2015 certified.