Absolute quantified peptides or AQUA peptides are used as internal references or peptide standards (absolute quantified reference peptides) for protein quantification (protein quantitation) in mass spectrometry-based proteomics, e.g. for selected reaction monitoring (SRM), multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM).
Absolute Quantified Peptides
Our proprietary absolute peptide quantification (peptide quantitation) method resulting in SpikeTides TQL (SpikeTides Tagged Quantified Labeled) overcomes the limitations of traditional methods such as limited accuracy and high costs:
- JPT’s QTag is a small chemical tag attached to the peptide
- QTag is released by trypsin and does not interfere with measurements
- Peptide quantification via HPLC-UV or HPLC-MS and UV
- Robust, reproducible & accurate method (variation CV= 5%)
- Validated in numerous peer-reviewed papers
- Lower cost than traditional methods
- Request small aliquots
More Proteomics Peptides
- Peptide Pools for Targeted Proteomics - SpikeMix™
- Reference Peptides for Targeted Proteomics - SpikeTides™
- Clinical Proteomics Peptides TQL_Plus
Comparison of Absolute Quantified Peptides
| SPIKETIDES™_TQL | SPIKETIDES™_TQL PLUS | |
|---|---|---|
| Quantification by HPLC-UV | X | X |
| Quantification by end-user possible | X | |
| Amount | 10 - 150nmol | 10 -150nmol |
| Aliquots | 1 - 10nmol/aliquot | 1 - 10nmol/aliquot |
| Purity | purified | > 95% guaranteed |
| Isotopical purity | 99% | 99% |
| Incoming material inspection | X | X |
| Vendor qualification | X | X |
| Batch documentation & CoA | X | X |
| Optional: certified vials | X | |
| Optional: additional QC methods | X |
Select your Absolute Quantified Peptides
1. SpikeTides™_TQ – absolute quantified, unlabeled peptides
SpikeTides™_TQ (Tagged Quantified) are quantified, proteotypic peptides with a C-terminal QTag that is cleaved by tryptic digestion.
- Amount: 10 - 150nmol
- Purity: Purified
- Application: Absolute protein quantification
- Validated pooling service available
2. SpikeTides™_TQL – absolute quantified, SIL peptides
SpikeTides™_TQL (Tagged Quantified Labeled)are heavy labeled, absolute quantified, proteotypic peptides with a C-terminal QTag that is cleaved by tryptic digestion.
- Amount: 10 - 150nmol
- Purity: Purified
- Application: Absolute protein quantification
- Validated pooling service available
3. SpikeTides™_Q_AAA & QL_AAA
SpikeTides™ Q_AAA (Quantified by AAA) & QL_AAA (Quantified by AAA and Labeled) are quantified without the QTag but with classical amino acid analysis (AAA).
Please inquire for your Maxi SpikeTides™ Q & QL_AAA via email!
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