Absolute quantified peptides are used as internal references or peptide standards (absolute quantified reference peptides) for protein quantification (protein quantitation) in mass spectrometry-based proteomics, e.g. for selected reaction monitoring (SRM), multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM).
Select your Absolute Quantified Peptides:
Our proprietary absolute peptide quantification (peptide quantitation) method overcomes the limitations of traditional methods such as limited accuracy and high costs:
- JPT’s QTag is a small chemical tag attached to the peptide
- QTag is released by trypsin and does not interfere with measurements
- Peptide quantification via HPLC-UV or HPLC-MS and UV
- Robust, reproducible & accurate method (variation CV= 5%)
- Validated in numerous peer-reviewed papers
- Lower cost than traditional methods
Also available:
- Peptide Pools for Targeted Proteomics - SpikeMix™
- Reference Peptides for Targeted Proteomics - SpikeTides™
- Clinical Proteomics Peptides TQL_Plus
Comparison of Absolute Quantified Peptides:
SPIKETIDES™_TQL | SPIKETIDES™_TQL PLUS | |
---|---|---|
Quantification by HPLC-UV | X | X |
Quantification by end-user possible | X | |
Amount | 5 x 1nmol | 5 x 10nmol |
Isotopical purity | 99% | 99% |
Incoming material inspection | X | X |
Vendor qualification | X | X |
Batch documentation & CoA | X | X |
Optional: certified vials | X | |
Optional: additional QC methods | X |
1. SpikeTides™_TQ – absolute quantified, unlabeled peptides
SpikeTides™_TQ are quantified, proteotypic peptides with a C-terminal QTag that is cleaved by tryptic digestion.
- Amount: 5 x 1 nmol/target peptide
- Purity: Purified
- Application: Absolute protein quantification
- Validated pooling service available
2. SpikeTides™_TQL – absolute quantified, SIL peptides
SpikeTides™_TQL are heavy labeled, absolute quantified, proteotypic peptides with a C-terminal QTag that is cleaved by tryptic digestion.
- Amount: 5 x 1 nmol/target peptide
- Purity: Purified
- Application: Absolute protein quantification
- Validated pooling service available
3. Maxi SpikeTides TQ & TQL
Maxi SpikeTides™ TQ & TQL are synthesized by standard resin-based custom peptide synthesis instead of high-throughput SPOT synthesis and can therefore be adjusted to your specifications.
Maxi SpikeTides™ TQ & TQL enable:
- Longer peptides
- Larger amounts per peptide
- Guaranteed purity for each peptide
- Classical AAA quantification of peptides
- Validated pooling service available
Please inquire for your Maxi SpikeTides™ via email!
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Quality Assurance
All production is ISO 9001:2015 certified.