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PepSpots™

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N-terminus	Acetyl (blocked)	Free amine	Other

We recommend N-terminal acetylation for peptide scans because: (1) peptides are more stable to degradation, (2) the uncharged N-ac better represents the region in the native antigen than a charged NH₃⁺-group.

Cys-Ser-Exchange	Yes

Note on Cys/Ser substitution: Cysteine containing peptides are sensitive to oxidation and cyclization. Therefore, substituting cysteine with serine residues increases the quality of the peptide. Cysteine is very rarely an essential residue in antibody epitopes.

Format	Peptide scan	Peptide length	Overlap
	Substitutional analysis	Comment
	Alanine scan
	Other

Application	Antibody epitope mapping	Linear epitope Discontinuous epitope
	Electroblotting is planned

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Interrested in:	Company information	Peptides
	Peptide Arrays	Immuno Tools
	Kinase Profiling Tools	Phosphatase Profiling Tools
	Protease Profiling Tools

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