Protease Substrate Identification
JPT provides highly comprehensive service to find substrates for your proteases using both peptides immobilized to glass surfaces (Protease Peptide Microarrays) and soluble peptides in 384-well microtiter plates (Protease Substrate Set). Discuss your needs with our scientists and simply send us your protease sample. JPT will perform all experiments and will deliver a data report including experimental details, fluorescence images and statistical analysis of results yielding preferences of amino acid residues in identified substrates and sequence motifs needed for efficient recognition of substrates.
Benefits of Protease Substrate Identification
- Use about 4 000 peptides available on peptide microarray
- Receive information about your protease substrates and cleavage sites within two weeks
- Get information about potential downstream targets of your protease
- Identify protease contaminations in your sample
- Minimal sample volume needed (200 µL assay volume per peptide microarray)
Selected References for Protease Substrate Identification
Chapter 2: Fluorescence-Based Biochemical Protease Assays Formats
Woelcke et al., A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery (2010)
Proteolytic Cleavage of Covalently Linked Cell Wall Proteins by Candida Albicans Sap9 and Sap10
Schild et al., Eukaryot. Cell (2010)
Related products and services
Protease Substrate Sets
Internally quenched (Dabcyl/EDANS) protease substrate collection ready-to-screen in microtiter plates.
ProteaseSpots™
Customized fluorescent peptides bound to cellulose discs in 96-well microtiter plates for protease profiling.
Phosphatase Substrate Identification
Phosphatase substrate identification service using JPT's peptide collections and microarrays
Kinase Substrate Identification
Kinase substrate identification service using JPT's peptide collections and microarrays
print