Phosphorylation Site Detector

JPT Peptide Technologies offers an efficient way to detect potential phosphorylation sites in kinase substrate proteins. Each Phosphorylation Site Detector represents a single peptide microarray on a glass slide containing an overlapping peptide scan through known known kinase subtrates (please refer to table below). Subsequent to incubation with target kinase in the presence of radioactive ATP, incorporated phosphate can be detected by autoradiography or phosphorimaging. Alternatively, phospho-specific antibody-based fluorescence readout is applicable for phosphopeptide detection.

Available Phosphorylation Site Detector microarrays

Phosphorylation Site-Detector 14-3-3 zeta
Peptide microarray displaying 79 peptides derived from a peptide scan (13 mers with 10 aa overlap) through 14-3-3 protein zeta/delta (Swiss-Prot ID: P63104) of Homo sapiens (Human)
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Phosphorylation Site-Detector AKT1
Peptide microarray displaying 157 peptides derived from a peptide scan through AKT1 of Homo sapiens (Human)
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Phosphorylation Site-Detector B-Myb
Peptide microarray displaying 345 peptides derived from a peptide scan through B-Myb of Homo sapiens (Human)
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Phosphorylation Site-Detector BCL 2
Peptide microarray displaying 79 peptides derived from a peptide scan through BCL 2 of Homo sapiens (Human)
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Phosphorylation Site-Detector c-jun
Peptide microarray displaying 160 peptides derived from a peptide scan through c-jun of Homo sapiens (Human)
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Phosphorylation Site-Detector CARS
Peptide microarray displaying 149 peptides derived from a peptide scan through CARS of Homo sapiens (Human)
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Phosphorylation Site-Detector CD5 antigen
Peptide microarray displaying 41 peptides derived from a peptide scan through CD5 antigene of Homo sapiens (Human)
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Phosphorylation Site-Detector Cdc25C
Peptide microarray displaying 155 peptides derived from a peptide scan through Cdc25C of Homo sapiens (Human)
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Phosphorylation Site-Detector CHK2
Peptide microarray displaying 178 peptides derived from a peptide scan through CHK2 of Homo sapiens (Human)
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Phosphorylation Site-Detector Connexin 43
Peptide microarray displaying 94 peptides derived from a peptide scan through Connexin 43 of Homo sapiens (Human)
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Phosphorylation Site-Detector Crystallin B
Peptide microarray displaying 82 peptides derived from a peptide scan through Crystallin B of Homo sapiens (Human)
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Phosphorylation Site-Detector Cyp11
Peptide microarray displaying 66 peptides derived from a peptide scan through Cyp11 of Homo sapiens (Human)
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Phosphorylation Site-Detector Cyp18.1
Peptide microarray displaying 149 peptides derived from a peptide scan through Cyp18.1 of Homo sapiens (Human)
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Phosphorylation Site-Detector Cyp18.2
Peptide microarray displaying 152 peptides derived from a peptide scan through Cyp18.2 of Homo sapiens (Human)
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Phosphorylation Site-Detector Cyp18.6
Peptide microarray displaying 52 peptides derived from a peptide scan through Cyp18.6 of Homo sapiens (Human)
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Phosphorylation Site-Detector Cyp19.2
Peptide microarray displaying 56 peptides derived from a peptide scan through Cyp19.2 of Homo sapiens (Human)
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Phosphorylation Site-Detector Cyp23
Peptide microarray displaying 66 peptides derived from a peptide scan through Cyp23 of Homo sapiens (Human)
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Phosphorylation Site-Detector Cyp23a
Peptide microarray displaying 67 peptides derived from a peptide scan through Cyp23a of Homo sapiens (Human)
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Phosphorylation Site-Detector Cyp33
Peptide microarray displaying 97 peptides derived from a peptide scan through Cyp33 of Homo sapiens
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Phosphorylation Site-Detector Cyp40
Peptide microarray displaying 120 peptides derived from a peptide scan through Cyp40 of Homo sapiens (Human)
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Phosphorylation Site-Detector Cyp57
Peptide microarray displaying 160 peptides derived from a peptide scan through Cyp57 of Homo sapiens (Human)
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Phosphorylation Site-Detector Cyp60
Peptide microarray displaying 170 peptides derived from a peptide scan through Cyp60 of Homo sapiens (Human)
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Phosphorylation Site-Detector Cyp73
Peptide microarray displaying 212 peptides derived from a peptide scan through Cyp73 of Homo sapiens (Human)
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Phosphorylation Site-Detector CYPA
Peptide microarray displaying 77 peptides derived from a peptide scan through CYPA of Homo sapiens (Human)
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Phosphorylation Site-Detector CypL1
Peptide microarray displaying 52 peptides derived from a peptide scan through CypL1 of Homo sapiens (Human)
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Phosphorylation Site-Detector CypL6
Peptide microarray displaying 100 peptides derived from a peptide scan through CypL6 of Homo sapiens (Human)
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Phosphorylation Site-Detector E2F
Peptide microarray displaying 143 peptides derived from a peptide scan through E2F of Homo sapiens (Human)
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Phosphorylation Site-Detector EpoR
Peptide microarray displaying 75 peptides derived from a peptide scan through EpoR of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBB12.6
Peptide microarray displaying 32 peptides derived from a peptide scan through FKBB12.6 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP12
Peptide microarray displaying 32 peptides derived from a peptide scan through FKBP12 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP13
Peptide microarray displaying 44 peptides derived from a peptide scan through FKBP13 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP19
Peptide microarray displaying 63 peptides derived from a peptide scan through FKBP19 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP22
Peptide microarray displaying 67 peptides derived from a peptide scan through FKBP22 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP23
Peptide microarray displaying 83 peptides derived from a peptide scan through FKBP23 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP25
Peptide microarray displaying 71 peptides derived from a peptide scan through FKBP25 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP36
Peptide microarray displaying 79 peptides derived from a peptide scan through FKBP36 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP37
Peptide microarray displaying 80 peptides derived from a peptide scan through FKBP37 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP38
Peptide microarray displaying 86 peptides derived from a peptide scan through FKBP38 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP44
Peptide microarray displaying 94 peptides derived from a peptide scan through FKBP44 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP51
Peptide microarray displaying 112 peptides derived from a peptide scan through FKBP51 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP52
Peptide microarray displaying 112 peptides derived from a peptide scan through FKBP52 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP63
Peptide microarray displaying 140 peptides derived from a peptide scan through FKBP63 of Homo sapiens (Human)
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Phosphorylation Site-Detector FKBP65
Peptide microarray displaying 144 peptides derived from a peptide scan through FKBP65 of Homo sapiens (Human)
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Phosphorylation Site-Detector GAB1
Peptide microarray displaying 342 peptides derived from a peptide scan through GAB1 of Homo sapiens (Human)
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Phosphorylation Site-Detector LKB1
Peptide microarray displaying 141 peptides derived from a peptide scan through LKB1 of Bos taurus
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Phosphorylation Site-Detector MDM2
Peptide microarray displaying 161 peptides derived from a peptide scan through MDM2 of Bos taurus
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Phosphorylation Site-Detector MeCP2
Peptide microarray displaying 159 peptides derived from a peptide scan through MeCP2 of Homo sapiens (Human)
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Phosphorylation Site-Detector NFATP
Peptide microarray displaying 305 peptides derived from a peptide scan through NFATP of Homo sapiens (Human)
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Phosphorylation Site-Detector NKTK
Peptide microarray displaying 208 peptides derived from a peptide scan through NKTK of Homo sapiens (Human)
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Phosphorylation Site-Detector Nucleophosmin1
Peptide microarray displaying 95 peptides derived from a peptide scan through Nucleophosmin1 of Homo sapiens (Human)
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Phosphorylation Site-Detector p53
Peptide microarray displaying 96 peptides derived from a peptide scan through p53 of Homo sapiens (Human)
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Phosphorylation Site-Detector PAK1
Peptide microarray displaying 181 peptides derived from a peptide scan through PAK1 of Homo sapiens (Human)
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Phosphorylation Site-Detector Par17
Peptide microarray displaying 48 peptides derived from a peptide scan through Par17 of Homo sapiens (Human)
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Phosphorylation Site-Detector Parvulin14
Peptide microarray displaying 41 peptides derived from a peptide scan through Parvulin14 of Homo sapiens (Human)
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Phosphorylation Site-Detector PDK1
Peptide microarray displaying 182 peptides derived from a peptide scan through PDK1 of Homo sapiens (Human)
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Phosphorylation Site-Detector Phas-I
Peptide microarray displaying 54 peptides derived from a peptide scan through Phas-I of Homo sapiens (Human)
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Phosphorylation Site-Detector Pin 1
Peptide microarray displaying 76 peptides derived from a peptide scan through Pin 1 of Homo sapiens (Human)
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Phosphorylation Site-Detector PTEN
Peptide microarray displaying 196 peptides derived from a peptide scan through PTEN of Digitalis lanata
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Phosphorylation Site-Detector PTMA
Peptide microarray displaying 50 peptides derived from a peptide scan through PTMA of Homo sapiens (Human)
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Phosphorylation Site-Detector PTPA
Peptide microarray displaying 78 peptides derived from a peptide scan through PTPA of homo sapiens (Human)
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Phosphorylation Site-Detector PTPN1
Peptide microarray displaying 142 peptides derived from a peptide scan through PTPN1 of Homo sapiens (Human)
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Phosphorylation Site-Detector RAD9
Peptide microarray displaying 96 peptides derived from a peptide scan through RAD9 of Homo sapiens (Human)
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Phosphorylation Site-Detector RAF1
Peptide microarray displaying 319 peptides derived from a peptide scan through RAF1 of Homo sapiens (Human)
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Phosphorylation Site-Detector RBL2
Peptide microarray displaying 377 peptides derived from a peptide scan through RBL2 of Homo sapiens (Human)
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Phosphorylation Site-Detector S6 protein
Peptide microarray displaying 80 peptides derived from a peptide scan through S6 protein of Homo sapiens (Human)
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Phosphorylation Site-Detector Tie 2
Peptide microarray displaying 172 peptides derived from a peptide scan through Tie 2 of Homo sapiens (Human)
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Applications for Phosphorylation Site Detector microarrays

  • Identification of kinase substrate peptides
  • Detection of potential phosphosites in substrate proteins
  • Determination of auto-phosphorylation sites
  • Identification of upstream kinases for your target protein
  • Elucidation of signal transduction pathways

Benefits of Phosphorylation Site Detector microarrays

  • Save time and material to determine phosphosites in proteins
  • Screen several hundred peptides economically
  • Learn more about your kinase/kinase target protein
  • Validate your results with purified peptides

Testimonials for Phosphorylation Site Detector:

"We have successfully applied JPT's peptide microarrays as a screening tool for potential kinases transfering phosphate to phosphorylation sites identified by mass spectrometry. The triplicate alignment as well as the thorough selection of controls enabled generation of high quality data confirming our in vivo data generated by means of affinity chromatographic procedures."
Dr. Marcelo P. Coba (The Wellcome Trust Sanger Institute, Cambridge, UK)

More testimonials under JPT Testimonials

Selected References for Phosphorylation Site Detector:

Peptide Arrays for Enzyme Profiling
Thiele et al., Methods Mol. Biol. (2009) - PMID: 19649588
Side Chain Specificity of ADP-Ribosylation by a Sirtuin

Fahie et al., FEBS Journal (2009) - PMID: 19895577

High-Density Peptide Microarrays for Reliable Identification of Phosphorylation Sites and Upstream Kinases
Thiele et al., Methods Mol Biol. (2009) - PMID: 19649595
Neurotransmitters Drive Combinatorial Multistate Postsynaptic Density Networks
Coba et al., Science Signaling (2009) - PMID: 19401593

More references under JPT Publications/Literature

Phosphorylation Site Detector: Microarrays Layout

Figure:
For quality control, four identical subarrays (including controls) are printed to each single microarray. All peptides are chemoselectively immobilized via their N-terminus. The hydrophilic linker moiety 1-amino-4,7,10-trioxa-13-tridecanamine succinimic acid is used as a spacer between the peptides and the glass surface.

Phosphorylation Site detector : Microarrays Details

Figure:
The full-length control proteins are covalently attached by non-selective immobilisation chemistry using the amino-function of the lysine-side chains. We can not guarantee that the proteins are natively folded.

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