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Phosphorylation Site Detector
JPT Peptide Technologies offers an efficient way to detect potential phosphorylation sites in kinase substrate proteins. Each Phosphorylation Site Detector represents a single peptide microarray on a glass slide containing an overlapping peptide scan through known known kinase subtrates (please refer to table below). Subsequent to incubation with target kinase in the presence of radioactive ATP, incorporated phosphate can be detected by autoradiography or phosphorimaging. Alternatively, phospho-specific antibody-based fluorescence readout is applicable for phosphopeptide detection.
Available Phosphorylation Site Detector microarrays
Applications for Phosphorylation Site Detector microarrays
- Identification of kinase substrate peptides
- Detection of potential phosphosites in substrate proteins
- Determination of auto-phosphorylation sites
- Identification of upstream kinases for your target protein
- Elucidation of signal transduction pathways
Benefits of Phosphorylation Site Detector microarrays
- Save time and material to determine phosphosites in proteins
- Screen several hundred peptides economically
- Learn more about your kinase/kinase target protein
- Validate your results with purified peptides
Testimonials for Phosphorylation Site Detector:
"We have successfully applied JPT's peptide microarrays as a screening tool for potential kinases transfering phosphate to phosphorylation sites identified by mass spectrometry. The triplicate alignment as well as the thorough selection of controls enabled generation of high quality data confirming our in vivo data generated by means of affinity chromatographic procedures."
Dr. Marcelo P. Coba (The Wellcome Trust Sanger Institute, Cambridge, UK)
More testimonials under JPT Testimonials
Selected References for Phosphorylation Site Detector:
Peptide Arrays for Enzyme Profiling
Thiele et al., Methods Mol. Biol. (2009)
Side Chain Specificity of ADP-Ribosylation by a Sirtuin
Fahie et al., FEBS Journal (2009)
High-Density Peptide Microarrays for Reliable Identification of Phosphorylation Sites and Upstream Kinases
Thiele et al., Methods Mol Biol. (2009)
Neurotransmitters Drive Combinatorial Multistate Postsynaptic Density Networks
Coba et al., Science Signaling (2009)
More references under JPT Publications/Literature
Figure:
For quality control, four identical subarrays (including controls) are printed to each single microarray. All peptides are chemoselectively immobilized via their N-terminus. The hydrophilic linker moiety 1-amino-4,7,10-trioxa-13-tridecanamine succinimic acid is used as a spacer between the peptides and the glass surface.
Figure:
The full-length control proteins are covalently attached by non-selective immobilisation chemistry using the amino-function of the lysine-side chains. We can not guarantee that the proteins are natively folded.
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Phosphorylation Site Detector Microarrays
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Our customer support team will assist with technical questions and product information:
e-mail: peptide@jpt.com
T: +49-30-6392-7878
T: +1 978 631 4225 (US/Canada)
JPT's Quality Assurance
All production is performed in JPT's head offices in Berlin, Germany according to ISO 9001:2008 standards
Applications
print © JPT Peptide Technologies GmbH
Top Indications
Main Applications
- Seromarker discovery & validation
- Immune monitoring (humoral & cellular immune responses)
- Vaccine target discovery
- Peptide lead identification & optimization
- Biomarker quantification by targeted proteomics
- Enzyme substrate identification
- Kinase profiling
- Phosphatase profiling
- Protease profiling
- Deacetylase & acetyltransferase profiling
Important Peptide Modifications
- Fluorescent labels (i.e. FITC, Carboxyfluorescein, Alexa, AMC, FRET)
- Peptide esters
- Internally quenched peptides (Abz/nitroTyr, EDANS/DABCYL, MCA/DNP labeling)
- Immunogenic peptides (i.e. MAPs, palmitinylation, Pam3Cys)
- Phosphopeptides (phosphorylated tyrosine, threonine or serine)
- Peptidomimetics (amide bond isosteres, non-natural amino acids, D amino acids peptides, etc.)
- Biotinylated peptides (N-terminal and C-terminal biotinylation as well as biotin attached via side chains – via special optimized linker / spacer)
- Post-translational modifications (PTMs) such as acetylation, methylation, phosphorylation, citrullination -e.g. acetyl lysine and acetyl arginine peptides, methyl lysine and methyl arginine, citrullin, lysine succinyl, butyryl, propyl
- Non-commercial building blocks for peptide synthesis available
- Isotopically labeled custom peptides (non-radioactive isotope, heavy peptides)
- Cyclic peptides (disulfide bridges, lactams, thioether-bridges, etc.)
- Site-directed conjugations of custom peptides with KLH, BSA, ovalbumine or other carriers
Overview of JPT's Peptide Products
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