Phosphorylation Site Detection

JPT provides a highly economical tool for determination of phosphorylation sites in substrate proteins. Overlapping peptides covering your substrate protein will be synthesized and immobilized onto glass surfaces or our premade phosphorylation site detectors can be used. Resulting peptide microarray will be screened using your kinase of interest in the presence of radioisotopically labelled ATP followed by phosphorimaging. JPT will perform all experiments and will deliver a data report including experimental details, phosphorimages and analysis of results yielding phosphorylation site(s).

Benefits of Phosphorylation Site Detection

• Select from more than 80 ready-to-screen Phosphorylation Site Detectors
• Create your customized Phosphorylation site detector microarray
• Receive information about phosphorylation sites within two weeks
• Minimal sample volume needed (200 µL assay volume per peptide microarray)
• Learn more about your kinase/kinase target protein

Applications for Phosphorylation Site Detection

• Detection of potential phosphosites in substrate proteins
• Determination of auto-phosphorylation sites
• Identification of upstream kinases for your target protein
• Elucidation of signal transduction pathways
• Get information about potential downstream targets of your kinase

Related products and services

Phosphorylation Site Detector
Ready-to-use PepStar™ peptide microarrays displaying overlapping peptide scans through known kinase substrate proteins
(full service available)

Kinase Peptide Microarrays
Peptide microarrays containing either annotated human phosphorylation site derived peptides or randomly generated peptide for identification of kinase substrates and profiling of kinase substrate specificities

Kinase Substrate Identification
Kinase substrate identification service using JPT's peptide collections and microarrays

Kinase Specificity Profiling
Comprehensive & knowledge based elucidation of substrate specificities

Phosphatase Substrate Identification
Phosphatase substrate identification service using JPT's peptide collections and microarrays

Protease Substrate Identification
Protease substrate identification service using JPT's peptide collections and microarrays

Testimonials for Phosphorylation Site Detection:

"With JPT´s peptide microarray platform, high- throughput kinase profiling using small amounts of cell lysate becomes feasible, providing access to the discovery and monitoring of novel biomarkers in Parkinson's Disease."
Jeremy Nichols, PhD (Parkinsons's Institute, Sunnyvale, CA, USA)

More testimonials under JPT Testimonials

Selected References for Phosphorylation Site Detection:

Peptide Arrays for Enzyme Profiling
Thiele et al., Methods Mol. Biol. (2009)
Side Chain Specificity of ADP-Ribosylation by a Sirtuin
Fahie et al., FEBS Journal (2009)
High-Density Peptide Microarrays for Reliable Identification of Phosphorylation Sites and Upstream Kinases
Thiele et al., Methods Mol Biol. (2009)
Neurotransmitters Drive Combinatorial Multistate Postsynaptic Density Networks
Coba et al., Science Signaling (2009)

More references under JPT Publications/Literature