Phosphatase Substrate Sets
JPT Peptide Technologies offers an efficient way to study phosphatase activities and substrate specificity. Our Phosphatase Substrate Sets each include a 384-well microtiter plate containing 360 phosphopeptides (250 pmol per well) derived from human phosphorylation sites together with 10 calibration standards. Subsequent to incubation with target phosphatase the released phosphate ions could be detected by complex formation to phosphate-binding dyes.
- Phosphatase Substrate Set (sequence information and microtiter plate layout)
- Tyrosine Phosphatase Substrate Set (sequence information and microtiter plate layout)
Benefits of Phosphatase Substrate Sets
- Screen 360 potential phosphatase substrates economically
- Save time for HTS-Assay development
- Learn more about the biology of your phosphatase
Applications for Phosphatase Substrate Sets
- Identification of phosphatase substrates
- Determination of phosphatase consensus sequences
- Identification of potential in vivo substrates/downstream targets
- Elucidation of signal transduction pathways
- Reliable identification of contaminating phosphatases
Selected References for Phosphatase Substrate Sets:
Characterization of the Catalytic Activity of the Membrane-anchored Metalloproteinase ADAM15 in Cell-based Assays
Maretzky et al., Biochem. J. (2009)
Protein-tyrosine Phosphatase H1 Controls Growth Hormone. Receptor Signaling and Systemic Growth
Pilecka et al., The journal of Biological Chemistry (2007)
More references under JPT Publications/Literature
Related products and services
Phosphatase Peptide Microarrays
Compilation of phosphatase substrate peptides on ready-to-use PepStar peptide microarrays.
Phosphatase Substrate Identification
Profiling service for phosphatases using high-content PepStar peptide microarrays.
Please inquire for customized Phosphatase Substrate Sets in microtiter plates tailor-made to your order.
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