Custom PepStar™ Peptide Microarrays

JPT Peptide Technologies applies its unique PepStar™ peptide microarray platform to generate customized peptide microarrays on glass slides.  Peptide microarrays (or peptide arrays) are used for characterization of enzymes , i.e. kinases, phosphatases and proteases as well as methyl, glycosyl, acetyl transferases and lysine deacetylases.
In addition, PepStar™ microarray technology also enables seromarker screening, humoral immune monitoring or elucidation of protein-protein interactions in general. The  
chemically synthesized (SPOT synthesis) peptides are covalentyl bound on glass slides via a flexible linker. Chemoselective coupling ensures that PepStar™ peptide microarrays display only directed and purified peptides. Hundreds of identical copies of each peptide microarray can be prepared at unmatched prices and delivery times. We include a validated application protocol for manual or automated incubation, an annotation file (.gal file) and a software tool to view the .gal file and basic analysis functions.

Applications for PepStar™ Peptide Microarrays:

  • Screening for substrates of orphan enzymes
  • Validation & optimization of enzyme substrates
  • Elucidation of signal transduction pathways
  • Detection of contaminating enzyme activities

    Application Notes:
    Download our Application Note on PepStar™ Peptide Microarrays:
    "Antibody Signatures Defined by High-Content Peptide Microarray Analyses"
    by Schutkowski et al
    Download our Application Note on our modular seromarker profiling service:

    "A Modular Approach for Epitope Discovery and High-Resolution Profiling of Humoral Immune Responses"
    by N. Pawlowski et al

Benefits of PepStar™ Peptide Microarrays:

  • Get hundreds of identical microarray copies from one peptide synthesis batch
  • Peptides are free of truncated sequences
  • Only small sample amounts are needed for incubation
  • Low background on glass surface
  • Sensitive fluorescence read-out
  • Parallel screening of thousands of potential peptide substrates
  • Learn more about the biology of your enzyme
  • We also provide individual custom substrate peptides for validation

Testimonials for PepStar™ Peptide Microarrays:

"The RV 144 HIV trial is considered as one of first successful HIV vaccine trials. It has become clear that the V2 loop of gp120 is an important site for immunogenicity and protection from HIV infection. The use of JPT's PepStar™ microarray technology has been very useful for the correlation of the clincial outcome with humoral immune responses. As have the cyclic peptides been from JPT to validate these findings!"
Jeff Currier, PhD, Walter Reed Army Institute, Rockville, Maryland, USA

"Over the past five years my research team has been active in the development of species-specific peptide arrays. Our ability to advance this technology into new research areas has been dependent on working with the highest quality of arrays. In this regard, JPT’s commitment to excellence has been a critical foundation for the success of our research program.  I have been consistently impressed with their customer service, technical expertise and professionalism. When it comes to peptide work there are no other options for our group…JPT is the best."
Scott Napper, PhD, Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Canada

More testimonials under JPT Testimonials

Selected References for PepStar™ Peptide Microarrays:

Nonreplicating Vaccines Can Protect African Green Monkeys From the Memphis 37 Strain of Respiratory Syncytial Virus
Eyles et al., The Journal of Infectious Disease (2013)
Serum Autoantibodies to Myelin Peptides Distinguish Acute Disseminated Encephalomyelitis From relapsing– Remitting Multiple Sclerosis
Van Haren et al., Multiple Sclerosis Journal (2013)
Functional Analysis of Centrosomal Kinase Substrates in Drosophila melanogaster Reveals a New Function of the Nuclear Envelope Component Otefin in Cell Cycle Progression
Habermann et al., Mol. Cell. Biol. (2012)

Zinc Metalloproteinase ProA Directly Activates Legionella pneumophila PlaC Glycerophospholipid:cholesterol Acyltransferase
Lang et al., J. of Biol. Chem (2012)

Diagnostic Peptide Discovery: Prioritization of Pathogen Diagnostic Markers Using Multiple Features
Carmona et al., PLOS ONE (2012)

Peptide arrays for kinome analysis: New opportunities and remaining challenges
Napper et al., Proteomics, Volume 11 (2011)

Systems Kinomics Demonstrates Congo Basin Monkeypox Virus Infection Selectively Modulates Host Cell Signaling Responses as Compared to West African Monkeypox Virus
Kindrachuk et al., Mol Cell Proteomics (2011)
Computational Analysis of High-density Peptide Microarray Data with Application from Systemic Sclerosis to Multiple Sclerosis
Hecker et. al, Autoimmunity Reviews (2011)
Humoral Reactome Profiles Using Peptide Microarray Chips
Valentini et al., Trends Immunol. (2010)

Related products and services

Epitope Discovery Service
Take advantage of our comprehensive Full Service (incl. incubation, analyses and detailed report)

Three-Step Seromarker Profiling Service

Comprehensive and modular seromarker discovery workflow ranging from high throughput screening and parallel verification of peptide hits for hundreds of samples to validation of results by robust peptide ELISA

RepliTope™ Catalog Microarrays
Peptide chips for mapping immunodominant regions in antigens
Phosphorylation Site Detector and Kinase Peptide Microarrays
Compilation of kinase substrate peptides on ready-to-use PepStar peptide microarrays
Peptide Microarray Assay Service
Profiling service using high content PepStar peptide microarrays
Phosphatase Peptide Microarrays
Compilation of phosphatase substrate peptides on ready-to-use PepStar peptide microarrays

Printer, PepStar™ Peptide Microarray and CD
Quote Request

Request a price for
PepStar™  Peptide Microarrays

Read about our off-the-shelf
RepliTope™ Peptide Microarrays

Contact us!

Our customer support team will assist with technical questions and product information:
e-mail: peptide@jpt.com
T: +49-30-6392-7878
T: +1 978 631 4225 (US/Canada)

JPT's Quality Assurance

All production is performed in JPT's head offices in Berlin, Germany according to ISO 9001:2008 standards

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