Kinase Substrate Identification

JPT provides highly comprehensive service to find substrates for your kinases using both peptides immobilized to glass surfaces (Kinase Peptides Microarrays) and soluble peptides in 384-well microtiter plates (Kinase Substrate Sets).
Discuss your needs with our scientists and simply send us your kinase sample. JPT will perform all experiments and will deliver a data report including experimental details and statistical analysis of results yielding consensus sequence motifs.

Benefits of Kinase Substrate Identification

• Select from more than 90 000 human peptides available on peptide microarrays
• Receive information about your kinase substrates and consensus sequence within two weeks
• Get information about potential downstream targets of your kinase
• Identify kinase contaminations in your sample
• Minimal sample volume needed (200 µL assay volume per peptide microarray)

Related products and services

Single Kinase Substrate Peptides
The substrates represent 13meric peptides derived from phosphorylation sites of human proteins.
Please select from the list of nearly 800 peptides currently available.

Kinase Peptide Microarrays
Peptide microarrays containing either annotated human phosphorylation site derived peptides or randomly generated peptide for identification of kinase substrates and profiling of kinase substrate specificities.

Kinase Substrate Sets
Compilation of kinase substrate peptides in ready-to-use microtiter plates.

Phosphatase Substrate Identification
Phosphatase substrate identification service using JPT's peptide collections and microarrays

Protease Substrate Identification
Protease substrate identification service using JPT's peptide collections and microarrays

Testimonials for Kinase Substrate identification:

"With JPT´s peptide microarray platform, high- throughput kinase profiling using small amounts of cell lysate becomes feasible, providing access to the discovery and monitoring of novel biomarkers in Parkinson's Disease."
Jeremy Nichols, PhD (Parkinsons's Institute, Sunnyvale, CA, USA)

Selected References for Kinase Substrate identification:

Computational Prediction and Experimental Verification of New MAP Kinase Docking Sites and Substrates Including Gli Transcription Factors
Whisenant et al, PLoS Computational Biology (2010)
Peptide Arrays for Enzyme Profiling
Thiele et al., Methods Mol. Biol. (2009)
Genome to Kinome: Species-Specific Peptide Arrays for Kinome Analysis
Jalal et al., Science Signaling (2009)
Identification of Candidate Substrates for Ectodomain Shedding by the Metalloprotease-Disintegrin ADAM8
Schutkowski et al., ChemBioChem (2005)

More references under JPT Publications/Literature