Kinase Peptide Microarrays

JPT Peptide Technologies has compiled a unique database of human phosphorylation sites. Peptides representing these phosphorylation sites are displayed on PepStar™ peptide microarrays.

The following peptide microarrays are available:

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Annotated Phosphosites-Kinase

This microarray containing 720 peptides derived from human phosphosites allows both, kinase substrate identification and consensus sequence determination. The peptides are presented as three spots per subarray within three subarrays per microarray. Each identified in vitro substrate peptide corresponds to a known protein, which represents a potential in vivo target of the studied kinase (microarray layout and sequence information).

Random Library Tyrosine-Kinase

This peptide microarray displays 1 536 peptides, containing one tyrosine residue in the center position. The peptides are presented as three spots per subarray within three subarrays per microarray. Screening of the complete or partial library results in the identification of peptidic substrates of the target tyrosine kinase (microarray layout and sequence information).

Random Library Threonine-Kinase

This peptide microarray displays 1 536 peptides, containing one threonine residue in the center position. The peptides are presented as three spots per subarray within three subarrays per microarray. Screening of the complete or partial library results in the identification of peptidic substrates of the target serine/threonine kinase (microarray layout and sequence information).

Random Library Serine-Kinase

This peptide microarray displays 1 536 peptides, containing one serine residue in the center position. The peptides are presented as three spots per subarray within three subarrays per microarray. Screening of the complete or partial library results in the identification of peptidic substrates of the target serine/threonine kinase (microarray layout and sequence information).

Related products and services

Single Kinase Substrate Peptides
The substrates represent 13meric peptides derived from phosphorylation sites of human proteins.
Please select from the list of nearly 800 peptides currently available.

Phosphorylation Site Detector
Ready-to-use PepStar™ peptide microarrays displaying overlapping peptide scans through known kinase substrate proteins
(full service available).

Kinase Substrate Sets
Compilation of kinase substrate peptides in ready-to-use microtiter plates.

Kinase Substrate identification
Profiling service for kinases using high-content PepStar™ peptide microarrays.

Testimonials for Kinase Peptide Microarrays

"We have successfully applied JPT's peptide microarrays as a screening tool for potential kinases transfering phosphate to phosphorylation sites identified by mass spectrometry. The triplicate alignment as well as the thorough selection of controls enabled generation of high quality data confirming our in vivo data generated by means of affinity chromatographic procedures."
Dr. Marcelo P. Coba (The Wellcome Trust Sanger Institute, Cambridge, UK)

Selected References for Kinase Peptide Microarrays:

Computational Prediction and Experimental Verification of New MAP Kinase Docking Sites and Substrates Including Gli Transcription Factors
Whisenant et al, PLoS Computational Biology (2010)
Peptide Arrays for Enzyme Profiling
Thiele et al., Methods Mol. Biol. (2009)
Genome to Kinome: Species-Specific Peptide Arrays for Kinome Analysis
Jalal et al., Science Signaling (2009)
Identification of Candidate Substrates for Ectodomain Shedding by the Metalloprotease-Disintegrin ADAM8
Schutkowski et al., ChemBioChem (2005)

More references under JPT Publications/Literature