PepSpots™ Peptide Arrays on Cellulose

JPT Peptide Technologies' PepSpots™ Peptide Arrays on cellulose membranes are synthesized with the unique SPOT peptide synthesis technique. SPOT synthesis is a fast and economic technique for parallel peptide synthesis on membrane supports. SPOT peptide synthesis was developed to give rapid and low-cost access to large numbers of peptides both as membrane bound developed a peptide synthesis technology (SPOT) to provide peptide arrays (PepSpots™) based on cellulose membranes for efficient and inexpensive B-cellantibody epitope mapping, protein-protein interaction studies etc. and as soluble peptide libraries (Micro-Scale Peptides). PepSpots™ peptide arrays are reliable and combine an easy experimental procedure, inexpensive equipment needs and a highly flexible array and library formatting. Subsequently, the mapped epitopes can be functionally characterized on the cellulose peptide array by rapid determination of key residues playing a definite role in the binding process. More than 100 scientific papers describing the application of this PepSpots™ peptide array technologies and the hundreds of successful epitope mapping and epitope characterization projects performed at JPT over the last decade distinguish PepSpot™ peptide arrays as the method of choice for fast and reliable antibody epitope mapping and characterization.

Applications for PepSpots™ Peptides on Cellulose

  • Antibody epitope mapping
  • Functional characterization of mapped epitopes (e.g substitutional or truncation analyses):
    Which amino acids are critical for binding?
    Which amino acids are so called "key-residues”?
  • Characterization of protein-protein contact sites, such as:
    • Receptor-ligand
    • Enzyme-substrate
    • Protein modules involved in signal transduction processes
    • Recognition sequences for heat shock proteins

Benefits of PepSpots™ Peptides on Cellulose

  • Rapid, economical, and accurate synthesis using robotics
  • Hydrophilic surface of cellulose membranes
  • Minimization of unspecific hydrophobic interactions with target molecule
  • Easy detection using standard ELISA protocols
  • Take advantage of our comprehensive All-In-One Epitope Discovery Service

Testimonials for PepSpots™ Peptides on Cellulose:

"Our teams at the Vrije Universiteit Brussel deal with a wide variety of projects in different areas of applied biological sciences. For many of them we are in need of peptide related tools and services ranging from peptide libraries and highly purified peptides to peptide arrays. In our search for an integrated and reliable provider we came across JPT. Meanwhile, we are collaborating with JPT for many years and are very satisfied with the relationship which led to several well received publications. Especially, their unique array technology helped us to enhance our knowledge on different protein protein interactions such as Chaperone-substrate recognition."
Prof. Joost W.H. Schymkowitz & Prof. Frederic Rousseau, VIB Switch Laboratory, Vrije Universiteit Brussel, Belgium

More testimonials under JPT Testimonials

Selected References for PepSpots™ Peptides on Cellulose:

Molecular Basis for the Unique Role of the AAA Chaperone ClpV in Type VI Protein Secretion
Pletrosluk et al., Journal of Biological Chemistry (2011) - PMID: 21733841
Protein Kinase CK2 Regulates the Formation and Clearance of Aggresomes in Response to Stress
Watabe et al., J. Cell Sci. (2011) - PMID: 21486957
HSP70 Natively and Specifically Associates with an N-terminal Dermcidin-derived Peptide That Contains an HLA-A*03 Antigenic Epitope
Stocki et al., J. Biol. Chem. (2011) - PMID: 21216960
A Robust Protocol to Map Binding Sites of the 14-3-3 Interactome: Cdc25C Requires Phosphorylation of Both S216 and S263 to bind 14-3-3
Chan et al., Mol. Cell. Proteomics. (2011) - PMID: 21189416
Biological Effects and Use of PrPSc- and PrP-Specific Antibodies Generated by Immunizing with Purified Full Length Native Mouse Prions
Petsch et al., J. Virol. (2011) - PMID: 21345946


More references under Publications/Literature

Comparison of PepSpots™ Peptides on Cellulose and PepStar™ Peptide Microarrays

PepSpots™ PepStar™
Peptides attached via C-terminus N-terminus
Peptides purified No Yes
Read-out via Chemiluminescence Fluorescence
Regeneration possible Yes No
Reorder possible from same synthesis No Yes, up to several hundred
Immobilization of protein controls possible No Yes, up to 8 controls
Incubation volume Depending on number of peptides (several ml) 200 - 300 µl
Usable of patient samples Limited Yes
Each peptide is displayed 1x 3x
Array surface Cellulose membrane Glass slide

Take advantage of our comprehensive All-In-One
Epitope Discovery Service

Send us an aliquot of your protein and the amino acid sequence of the binding partner and we will perform:

  • Synthesis of PepSpot™ peptides
  • Detection with your protein and data interpretation
  • Functional characterization of mapped epitopes
  • Complete and confidential documentation of results
PepSpots™ Peptides on Cellulose
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PepSpots™ Peptides on Cellulose

 

Read about our tailored
PepStar™  Peptide Microarrays

Contact us!

Please feel free to call or send us an email to peptide@jpt.com
+49-30-6392-7878
+1 978 631 4225 (US/Canada only)

JPT's Quality Assurance

All production is performed in JPT's head offices in Berlin, Germany according to ISO 9001:2008 standards

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