Benefits of PepMix™
- Equivalent or better stimulation of CD4 and CD8
- T-cell responses compared to whole protein antigens
- Simultaneous detection of CD4 and CD8 responses in a single sample
- Improved responses in stored blood and PBM cells compared to whole
protein antigens
Applications for PepMix™
- Immunostimulation of T-lymphocytes
- Monitoring of immune status during diseases
- Assessment of vaccine efficacy
Single Peptides
In addition to the mixes, peptides can also be ordered as single
peptide aliquots to validate the results found by the mixes. JPT Peptide
Technologies GmbH also offers customized PepMixes™ which will
be assembled in a high-throughput manner according to the customer's
demand for a variety of proteins. All peptides and mixes will be quality
controlled to ensure batch-to-batch reproducibility!
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Testimonials
"I have used JPT's peptide pools (PepMix) for years now, with
great satisfaction! First CMV derived peptides and now also EBV. So
far we have published data in one publication and plan several more
in the near future."
Anna Karin Lidehäll
(Uppsala University, Clin. Immunol., Uppsala, Sweden)
„The focus of the Clinical Immunology Group at the German Rheumatism
Research Centre is to study the role of cells and molecules in the
origin and progression of autoimmune and other diseases. In our hands
PepMixes™ were found to be effective in monitoring the immune
status of various patient populations but show also promise for the
development of novel immuno therapy approaches.”
Andreas Thiel, PhD
(German Rheumatism Research Centre, Clin. Immunol., Berlin, Germany)
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References:
- Massive Load of Functional Effector CD4+ and CD8+ T Cells against
Cytomegalovirus in Very Old Subjects, Myers et al.,
J Immunol. 179(6) (2007), p. 4283-4291 (abstract)
- Reconstitution of adenovirus-specific cell-mediated immunity
in pediatric patients after hematopoietic stem cell transplantation,
Myers et al., Bone Marrow Transplant. 39 (11) (2007), p. 677-686
(abstract)
- Monoculture-derived T lymphocytes specific for multiple viruses
expand and produce clinically relevant effects in immunocompromised
individuals, Leen at al., Nat. Med. 12(10) (2006), p. 1160-1166
(abstract)
- Results of a cytomegalovirus (CMV)-specific CD8+/interferon-
gamma+ cytokine flow cytometry assay correlate with clinical evidence
of protective immunity in patients with AIDS with CMV retinitis, Jacobson
et al., J. Infect. Dis. 189 (8) (2004), p. 1362–1373 (abstract)
- Functional Comparison of T Cells Recognizing Cytomegalovirus
pp65 and Intermediate-Early Antigen Polypeptides in Hematopoietic
Stem-Cell Transplant and Solid Organ Transplant Recipients, Lacey
et al., J. Infect. Dis. 194 (10) (2006), p. 1410–1421 (abstract)
- Acquisition of direct antiviral eff ector functions by CMV-specifi
c CD4+ T lymphocytes with cellular maturation, Casazza et al.,
J. Exp. Med. 203 (13) (2006), p. 2865–2877 (abstract)
- Protection from cytomegalovirus after transplantation is correlated
with immediate early 1–specific CD8 T cells, Bunde et al.,
Eur. J. Exp. Med. 201 (7) (2005), p. 1031-1036 (abstract)
- HLA type-independent generation of antigen-specific T cells
for adoptive immunotherapy, Hammer et al., Eur. J. Immunol. Vol.
35 (2005), p. 2250-2258 (abstract)
- Generation of cytomegalovirus (CMV)-specific T-lymphocytes using
protein-spanning pools of pp65-derived overlapping pentadecapeptides
for adoptive immunotherapy, Trivedi et al., Blood Vol. 105(7)
(2005) , p. 2793-2801 (abstract)
- Confirmation of Mycobacterium tubercolosis Infection by Flow
Cytometry after ex vivo Incubation of peripheral blood T Cells with
an ESAT-6-derived peptide pool, Tesfa et al., Cytometry Part
B (Clinical Cytometry) Vol. 60B (2004) p. 47-53 (abstract)
- CMV Antigen-Specific CD4+ and CD8+ T Cell IFN Expression and
Proliferation Responses in Healthy CMV-Seropositive Individuals,
Sinclair et al., Viral Immunol. Vol. 17(3) (2004), p. 445-454 (abstract)
- Recent Advances in the Development of HIV-1 Vaccines Using Replication-Incompetent
Adenovirus Vectors, Shiver and Emini, Annual Review of Medicine
Vol. 55 (2004), p. 355-372 (abstract)
- Mapping T cell epitopes by flow cytometry, Hoffmeister
et al., Methods Vol. 29(3) (2003), p. 270-281 (abstract)
- Cytomegalovirus (CMV) phosphoprotein 65 makes a large contribution
to shaping the T cell repertoire in CMV-exposed individuals,
Kern et al., J. Infect. Dis.Vol 185 (2002), p. 1709-1716 (article)
- Use of overlapping peptide mixtures as antigens for cytokine
flow cytometry, Maecker et al., J. Immunol. Methods Vol. 255
(2001), p. 27-40 (abstract)
- Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning
pools of overlapping pentadecapeptides, Kern et al., Eur. J.
Immunol. Vol 30 (2000), p. 1676-1682 (abstract)
- Putative immunodominant Human Immunodeficiency Virus-specific
CD8+ T-cell responses cannot be predicted by major histocompatibility
complex class I haplotype, Betts et al., J. Virol. Vol 74 (2000),
p. 9144-9151 (article)
- Identification of T-cell epitopes using ELISpot and peptide
pool arrays, Tobery and Caulfield, Methods in Molecular Medicine
Vol. 94: Molecular Diagnosis of Infectious Deseases, 2/e; Edited by
J.Decker and U. Reischl; Humana Press Inc, Totowa, NJ, p. 121-132
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