Phosphorylation Site Detector

JPT Peptide Technologies GmbH offers an efficient way to detect potential phosphorylation sites in kinase substrate proteins. Each Phosphorylation Site Detector represents a single peptide microarray on a glass slide containing an overlapping peptide scan through a protein in triplicates (three identical subarrays ensuring data quality). Subsequent to incubation with target kinase in the presence of radioactive ATP, incorporated phosphate can be detected by autoradiography or phosphorimaging. Alternatively, phospho-specific antibody-based fluorescence readout is applicable for phosphopeptide detection.

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> Array layout
> Subarray layout including control proteins

Benefits - Phosphorylation Site Detector microarrays

Save time and material to determine phosphosites in proteins
Screen several hundred peptides economically
Learn more about your kinase/kinase target protein

Applications - Phosphorylation Site Detector microarrays

Identification of kinase substrate peptides
Detection of potential phosphosites in substrate proteins
Determination of auto-phosphorylation sites
Identification of upstream kinases for your target protein
Elucidation of signal transduction pathways

References:

Proteomic Analysis of in Vivo Phosphorylated Synaptic Proteins, Collins et al., J. Biol. Chem., Vol. 280:7 (2005), p. 5972-5982 (abstract)

Available Phosphorylation Site Detector microarrays

Protein	Organism	ID-Code	Primary accession number	Database	Pdf file - Sequences - Chip-design	Peptides per subarray	Scan type*
MBP	bos taurus	MBP_BOVIN	P02687	Swiss-Prot		74	13/11
Histone H1	bos taurus	H11_BOVIN	P02253	Swiss-Prot		47	13/11
Tau	homo sapiens	TAU_HUMAN	P10636	Swiss-Prot		373	13/11
beta-casein	homo sapiens	CASB_HUMAN	P05814	Swiss-Prot		67	13/10
Pin 1	homo sapiens	PIN1_HUMAN	Q13526	Swiss-Prot		76	13/11
Parvulin14	homo sapiens	PIN4_HUMAN	Q9Y237	Swiss-Prot		60	13/11
Pin 1	digitalis lanata	PIN1_DIGLA	Q9LEK8	Swiss-Prot		54	13/11
NEK 2	homo sapiens	NEK2_HUMAN	P51955	Swiss-Prot		217	13/11
C-Nap1(CEP2)	homo sapiens	CEP2_HUMAN	Q9BV73	Swiss-Prot		194	13/10
FKBP12	homo sapiens	FKB1_HUMAN	P62942	Swiss-Prot		48	13/11
FKBB12.6	homo sapiens	FKBB_HUMAN	P68106	Swiss-Prot		48	13/11
FKBP13	homo sapiens	FKB2_HUMAN	P26885	Swiss-Prot		66	13/11
FKBP19	homo sapiens	FK11_HUMAN	Q9NYL4	Swiss-Prot		95	13/11
FKBP22	homo sapiens	FK14_HUMAN	Q9NWM8	Swiss-Prot		100	13/11
FKBP23	homo sapiens	FKB7_HUMAN	Q9Y680	Swiss-Prot		124	13/11
FKBP25	homo sapiens	FKB3_HUMAN	Q00688	Swiss-Prot		107	13/11
FKBP36	homo sapiens	FKB6_HUMAN	O75344	Swiss-Prot		106	13/10
FKBP37	homo sapiens	AIP_HUMAN	O00170	Swiss-Prot		107	13/10
FKBP38	homo sapiens	FKB8_HUMAN	Q14318	Swiss-Prot		115	13/10
Tie 2	homo sapiens	TIE2_HUMAN	Q02763	Swiss-Prot		172	13/11
FKBP44	homo sapiens	AIPL_HUMAN	Q9NZN9	Swiss-Prot		125	13/10
FKBP51	homo sapiens	FKB5_HUMAN	Q13451	Swiss-Prot		149	13/10
FKBP52	homo sapiens	FKB4_HUMAN	Q02790	Swiss-Prot		150	13/10
CYPA	homo sapiens	PPIA_HUMAN	P62937	Swiss-Prot		77	13/11
FKBP65	homo sapiens	FK10_HUMAN	Q96AY3	Swiss-Prot		144	13/9
FKBP135	homo sapiens	Q9Y4D0	Q9Y4D0	TrEMBL		205	13/7
Cyp18.1	homo sapiens	PPIL3_HUMAN	Q9H2H8	TrEMBL		149	13/12
Cyp18.2	homo sapiens	Q9Y536_HUMAN	Q9Y536	TrEMBL		152	13/12
Cyp18.6	homo sapiens	PPIL3_HUMAN	Q9BXZ1	TrEMBL		153	13/12
Cyp19.2	homo sapiens	PPIH_HUMAN	O43447	Swiss-Prot		165	13/12
Cyp11	homo sapiens	PPIF_HUMAN	P30405	Swiss-Prot		195	13/12
Cyp23	homo sapiens	PPIB_HUMAN	P23284	Swiss-Prot		196	13/12
RBL2	homo sapiens	NP_005602	NP_005602	NCBI		377	13/10
NFATP	homo sapiens	NP_775114	NP_775114	NCBI		305	13/10
p53	homo sapiens	NP_000537	NP_000537	NCBI		96	13/9
B-Myb	homo sapiens	NP_002457	NP_002457	NCBI		345	13/11
GAB1	homo sapiens	NP_002030	NP_002030	NCBI		342	13/11
PTMA	homo sapiens	NP_002814	NP_002814	NCBI		50	13/11
RAD9	homo sapiens	NP_004575	NP_004575	NCBI		96	13/9
Connexin 43	homo sapiens	NP_000156	NP_000156	NCBI		94	13/9
PTEN	homo sapiens	NP_000305	NP_000305	NCBI		196	13/11
c-jun	homo sapiens	NP_002219	NP_002219	NCBI		160	13/11
MDM2	homo sapiens	NP_002383	NP_002383	NCBI		161	13/10
crystallin B	homo sapiens	NP_001876	NP_001876	NCBI		82	13/11
EpoR	homo sapiens	NP_000112	NP_000112	NCBI		75	13/10
CD5 antigene	homo sapiens	NP_055022	NP_055022	NCBI		41	13/11
Cdc25C	homo sapiens	NP_001781	NP_001781	NCBI		155	13/10
PTPN1	homo sapiens	NP_002818	NP_002818	NCBI		142	13/10
Phas-I	homo sapiens	NP_004086	NP_004086	NCBI		54	13/11
S6 protein	homo sapiens	NP_001001	NP_001001	NCBI		80	13/10
E2F	homo sapiens	NP_005216	NP_005216	NCBI		143	13/10
Nucleophosmin 1	homo sapiens	NP_002511	NP_002511	NCBI		95	13/10
14-3-3 zeta	homo sapiens	NP_003397	NP_003397	NCBI		79	13/10
BCL 2	homo sapiens	NP_004313	NP_004313	NCBI		79	13/11
MeCP2	homo sapiens	NP_004983	NP_004983	NCBI		159	13/10
AKT1	homo sapiens	NP_005154	NP_005154	NCBI		157	13/10
RAF1	homo sapiens	NP_002871	NP_002871	NCBI		319	13/11
LKB1	homo sapiens	NP_000446	NP_000446	NCBI		141	13/10
PAK1	homo sapiens	AAC24716	AAC24716	NCBI		181	13/10
CHK2	homo sapiens	NP_009125	NP_009125	NCBI		178	13/10
PDK1	homo sapiens	NP_002604	NP_002604	NCBI		182	13/10

*The first number X indicates the total number of amino acids of the peptide-sequence, whereas the second number Y indicates the number of overlapping amino acids.

Figure:
For quality control three identical subarrays (including controls) are printed to each single microarray. All peptides are chemoselectively immobilized via their N-terminus. The hydrophilic linker moiety 1-amino-4,7,10-trioxa-13-tridecanamine succinimic acid is used as a spacer between the peptides and the glass surface.

Figure:
The full length control proteins are covalently attached by non-selective immobilisation chemistry using the amino-function of the lysine-side chains. We can not guarantee that the proteins are natively folded.

	Protein	Company	Cat #
VIII	Tau-Protein	Sigma	T-9392
VII	β-Casein	Sigma	C-6905
VI	α-Casein	Sigma	C-7891
V	MBP	Biomol- Upstate	13-104
IV	Histon 4	Biomol- Upstate	14-412
III	Histon 3	Biomol- Upstate	14-411
II	Histon 2A	Biomol- Upstate	14-409
I	Histon 1	Biomol- Upstate	14-155

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