PepStar™

JPT Peptide Technologies applies its unique PepStar™ peptide microarray platform to generate customized and high content off-the-shelf peptide microarrays on glass slides for studying enzyme specificities and protein interactions. In addition, PepStar™ technology also enables characterization of enzyme target families such as kinases, phosphatases and proteases as well as transferases and deacetylases.
The PepStar™ technology is a unique peptide microarray platform combining JPT’s custom peptide synthesis expertise and SPOT™ synthesis capabilities for ultra high throughput synthesis of peptides. Soluble peptides are deposited on glass slides by non-contact printing. Chemoselective coupling generates microarrays of directed and covalently attached peptides. Multiple copies of peptide microarrays can be prepared at unmatched prices and turnaround times.
JPT Peptide Technologies produce microarrays for standard applications as well as custom designed chips.

Benefits:

• Get hundreds of identical microarray copies from one
  peptide synthesis via economical SPOT™ technology
• Save time for HTS assay setup
• Screen thousands of potential peptide substrates economically
• Learn more about the biology of your enzyme

Applications:

• Mapping of immunodominant regions in antigens
• Identification of substrates for orphan enzymes
• Optimization of known enzyme substrates
• Elucidation of signal transduction pathways
• Reliable detection of contaminating enzyme activities

References:

• Validation of peptide epitope microarray experiments and extraction of quality data, Nahtman et al., J. Immunol. Methods 328 (2007), p. 1–13 (abstract)

• Prion recognition elements govern nucleation, strain
  specificity and species barriers, Tessier & Lindquist,
  Nature (2007) (abstract)

• High-Content Peptide Microarrays for Deciphering Kinase Specificity and Biology, Schutkowski et al., Angew. Chem.
  Int. Ed., Volume 43, Issue 20 (2004), p. 2671 - 2674
  (abstract)

• Profiling of generic anti-phosphopeptide antibodies and kinases with peptide microarrays using radioactive and fluorescence-based assays, Panse et al., Molecular
  Diversity, Vol. 8 (2004), p. 291 –299 (abstract)

• Peptide arrays: from macro to micro, Reimer et al., Curr.
  Opin. Biotechnol. Vol. 13 (2002), p. 315-320 (abstract)

Peptide chips for mapping immunodominant regions in antigens:
RepliTope™

Compilation of kinase substrate peptides on ready-to-use PepStar peptide microarrays:
Phosphorylation Site Detector and Kinase Peptide Microarrays

Profiling service for kinases using high content PepStar peptide microarrays:
Kinase Profiling Service

Compilation of phosphatase substrate peptides on ready-to-use PepStar peptide microarrays:
Phosphatase Peptide Microarrays

Profiling service for phosphatases using high content PepStar peptide microarrays:
Phosphatase Profiling Service

Compilation of protease substrate peptides on ready-to-use PepStar peptide microarrays:
Protease Peptide Microarrays

Profiling service for proteases using high content PepStar peptide microarrays:
Protease Profiling Service