RepliTope™

JPT provides a novel and highly economical tool to detect immunodominant regions in target antigens using overlapping peptide scans displayed on glass slides. Our off-the-shelf and customized RepliTope™ peptide microarrays represent a reliable and efficient source for the characterization of potential therapeutic antibodies, the identification of novel biomarkers, and the elucidation of protein/protein and enzyme/substrate interactions in general.
Moreover, RepliTope™ is applicable for monitoring humoral immune responses using sera in preclinical and clinical trials.

Quote Request (off-the-shelf and customized RepliTope™)

> Available off-the-shelf RepliTope™
> Exemplaric layout for off-the-shelf RepliTope™

Benefits of RepliTope™

Flexible and economical access to many identical peptide microarrays

Ready-to-use RepliTope™ peptide microarrays through
dozens of relevant antigens available within days
Production of customized peptide scans on chips within
less than 3 weeks
Optimized surfaces and built-in quality control
Fully compatible with standard ELISA protocols

Applications of RepliTope™

Reliable epitope mapping using minimal amounts of antibody

Efficient profiling of antibody collections directed against
the same antigen
In-vitro study of anti-antibody / neutralizing antibody action
Development of phosphorylation dependent antibodies
Monitoring of side effects of protein drugs during clinical trials
Identification of biomarkers

All-in-one Service

Send us your antibodies and the amino acid sequence of target antigen and we will perform:

Sequence design and peptide scan synthesis
Printing of peptide microarrays
Functional characterization of mapped epitopes
Complete and confidential documentation of results

References:

Validation of peptide epitope microarray experiments and extraction of quality data, Nahtman et al., J. Immunol. Methods 328 (2007), p. 1–13 (abstract)

PepMix Single Peptides Laboratory

Off-the-Shelf RepliTope™ peptide microarrays

Protein	Source	Primary accession number	Database	Peptides per subarray	Scan type (X/Y) *
Cryptic protein	Bacillus anthracis	AAA22636	NCBI	46	15/11
Protective antigen precursor	Bacillus anthracis	AAA22637	NCBI	189	15/11
Small acid-soluble protein (gamma type)	Bacillus anthracis (strain BC)	AAZ79419	NCBI	21	15/11
BKV (coat protein VP1)	BK polyomavirus	P14996	Swiss-Prot	88	15/11
BKV (large T antigen)	BK polyomavirus	P14999	Swiss-Prot	170	15/11
Capsid protein	Dengue virus type 2	AAA42941 region: 2-114	NCBI	26	15/11
Membrane glycoprotein	Dengue virus type 2	AAA42941 region: 206-280	NCBI	16	15/11
Envelope protein	Dengue virus type 2	AAA42941 regionN: 281-775	NCBI	121	15/11
Nonstructural protein 1	Dengue virus type 2	AAA42941 region: 776-1127	NCBI	86	15/11
Nonstructural protein 3	Dengue virus type 2	AAA42941 region: 1476-2093	NCBI	152	15/11
EBV (LMP2a/LMP2b)	Ebstein-Barr virus	P13285	Swiss-Prot	122	15/11
HCV (NS3)	Hepatitis C virus	P26663	Swiss-Prot	150	15/11
HIV-1 (gag consensus motif)	HIV	n.a.	n.a.	139	15/11
HIV-1 env (gp120 protein + signal peptide)	HIV	P04578 region: 1-511	Swiss-Prot	164	15/11
HIV-1 env (gp41)	HIV	P04578 region: 512-856	Swiss-Prot	116	15/11
NY-ESO-1	Homo sapiens	P78358	Swiss-Prot	43	15/11
WT1 (WT33)	Homo sapiens	P19544	Swiss-Prot	110	15/11
Melanoma antigen (OIP4)	Homo sapiens	P78395	Swiss-Prot	125	15/11
IE-1	Human cytomegalovirus	P13202	Swiss-Prot	120	15/11
Secreted antigen 85-B FBPB	M. tuberculosis (H37Rv)	CAB10044	NCBI	105	15/12
MPT70	M. tuberculosis (H37Rv)	CAA98373	NCBI	61	15/12
Immunogenic protein MPT63	M. tuberculosis (H37Rv)	CAB06500	NCBI	49	15/12
Immunogenic protein MPT64	M. tuberculosis (H37Rv)	CAA98382	NCBI	72	15/12
Probable isocitrate dehydrogenase [NADP] ICD1	M. tuberculosis (H37Rv)	CAA17111	NCBI	133	15/12
Low molecular weight protein antigen 7 ESXH	M. tuberculosis (H37Rv)	CAA17363	NCBI	28	15/12
Low molecular weight T-cell antigen TB8.4	M. tuberculosis (H37Rv)	NP_215690	NCBI	33	15/12
Probable isocitrate dehydrogenase [NADP] ICD2	M. tuberculosis (H37Rv)	CAA16247	NCBI	245	15/12
S protein	SARS coronavirus urbani	AAP13441	NCBI	311	15/11
E protein	SARS coronavirus urbani	AAP13443	NCBI	17	15/11
M protein	SARS coronavirus urbani	AAP13444	NCBI	53	15/11
N protein	SARS coronavirus urbani	AAP13445	NCBI	103	15/11
Envelope glycoprotein E	West Nile virus (strain NY99-flamingo 382-99)	AAF20092 region: 291-791	NCBI	123	15/11

*The first number X indicates the peptide length of a single peptide of the peptide scan, whereas the second number Y indicates the number of overlapping amino acids.

Figure:
For quality control three identical subarrays (including controls) are printed to each single microarray. All peptides are chemoselectively immobilized via their N-terminus. The hydrophilic linker moiety 1-amino-4,7,10-trioxa-13-tridecanamine succinimic acid is used as a spacer between the peptides and the glass surface.

Figure:
The control antibodies are covalently attached by non-selective immobilisation chemistry using the amino-function of the lysine-side chains. We do not guarantee that the antibodies are natively folded.

	Protein	Company	Cat #
IV	IgG Human	Sigma	I - 4506
III	IgE Human	Diatec.com	7050 - A10
II	IgM Human	Sigma	I - 8260
I	IgA Human	Sigma	I - 1010