PepStar™ Peptide Microarrays

JPT Peptide Technologies applies its unique PepStar™ peptide microarray platform to generate high-content and multiwell peptide microarrays on glass slides. Peptide arrays (or peptide chips) are used for biomarker discovery, immuno monitoring, multiplexed epitope mapping or for detection and validation of protein interactions. In addition, PepStar™ peptide microarray technology also enables characterization of enzyme target families such as kinases, phosphatases and proteases as well as methyl, glycosyl, acetyl transferases and lysine deacetylases.
Chemical peptide synthesis is followed by peptide immobilization on glass slides via a flexible linker. By chemoselective coupling we generate microarrays displaying  directed and covalently attached peptides. Multiple identical copies of each peptide microarray can be prepared with flexible layouts at unmatched prices and turnaround times. We provide application protocols for manual and automated incubation and fluorescence read-out with all our peptide microarrays.

Applications for PepStar™ Peptide Microarrays:

  • Mapping of immunodominant regions in antigens
  • Identification of substrates for orphan enzymes
  • Optimization of known enzyme substrates
  • Elucidation of signal transduction pathways
  • Reliable detection of contaminating enzyme activities


Application Notes:
Download our Application Note on systematic mimotope optimization using JPT's peptide array technology
"Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic antibody IMAB362"
by Daneschar et al.
Download our Application Note on PepStar™ Peptide Microarrays:
"Antibody Signatures Defined by High-Content Peptide Microarray Analyses"
by Schutkowski et al.
Download our Application Note on our modular seromarker profiling service:

"A Modular Approach for Epitope Discovery and High-Resolution Profiling of Humoral Immune Responses"
by N. Pawlowski et al.

Benefits of PepStar™ Peptide Microarrays:

  • Get hundreds of identical microarray copies from one peptide synthesis
  • Peptides are free of truncated sequences
  • Small amounts of your precious samples are needed for incubation
  • Low background on glass surface
  • Read-out via fluorescence
  • Save time for HTS assay setup
  • Screen thousands of potential peptide substrates economically
  • Learn more about the biology of your enzyme
  • Obtain Peptide ELISAs and individual custom peptides for validation
  • Flexible peptide microarray layout, e.g. for automated incubation and multiwell microarray incubation
  • Modular incubation and assay service incl. data evaluation available

Testimonials for PepStar™ Peptide Microarrays:

"The RV 144 HIV trial is considered as one of first successful HIV vaccine trials. It has become clear that the V2 loop of gp120 is an important site for immunogenicity and protection from HIV infection. The use of JPT's PepStar™ microarray technology has been very useful for the correlation of the clincial outcome with humoral immune responses. As have the cyclic peptides been from JPT to validate these findings!"
Jeff Currier, PhD, Walter Reed Army Institute, Rockville, Maryland, USA

"Over the past five years my research team has been active in the development of species-specific peptide arrays. Our ability to advance this technology into new research areas has been dependent on working with the highest quality of arrays. In this regard, JPT’s commitment to excellence has been a critical foundation for the success of our research program.  I have been consistently impressed with their customer service, technical expertise and professionalism. When it comes to peptide synthesis there are no other options for our group…JPT is the best."
Scott Napper, PhD, Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Canada

More testimonials under JPT Testimonials

Selected References for PepStar™ Peptide Microarrays:

Abstracts available from PubMed: www.ncbi.nlm.nih.gov/pubmed

Peptide Microarrays Enable Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic Antibody IMAB362
Schnatbaum et al., Biotechnol J. (2014) - PMID: 24497417
Peanut Oral Immunotherapy Modifies IgE and IgG4 Responses to Major Peanut Allergens
Vickery et al., J Allergy Clin Immunol. (2013) - PMID: 23199605
Plasma IgG to Linear Epitopes in the V2 and V3 Regions of HIV-1 gp120 Correlate with a Reduced Risk of Infection in the RV144 Vaccine Efficacy Trial
Gottardo et al., PLoS One. (2013) - PMID: 24086607
Nonreplicating Vaccines Can Protect African Green Monkeys From the Memphis 37 Strain of Respiratory Syncytial Virus

Eyles et al., The Journal of Infectious Disease (2013) - PMID: 23596321
Serum Autoantibodies to Myelin Peptides Distinguish Acute Disseminated Encephalomyelitis From relapsing– Remitting Multiple Sclerosis

Van Haren et al., Multiple Sclerosis Journal (2013) - PMID: 23612879
Neutralization of Porcine Endogenous Retrovirus by Antibodies Against the Membrane-Proximal External Region of the Transmembrane Envelope Protein

Wächter et al., Journal of General Virology (2013) - PMID: 23223617
Characterization of Humoral and Cellular Immune Responses Elicited by a Recombinant Adenovirus Serotype 26 HIV-1 Env Vaccine in Healthy Adults (IPCAVD 001)

Barouch et al., J. Immunol. (2013) - PMID: 23125443


More references under JPT Publications/Literature

PepStar

Related products and services

Three-Step Seromarker Profiling Service

Comprehensive and modular seromarker discovery workflow ranging from high throughput screening and parallel verification of peptide hits for hundreds of samples to validation of results by robust peptide ELISA

RepliTope™ Catalog Microarrays
Peptide chips for mapping immunodominant regions in antigens
Kinase Peptide Microarrays
Compilation of kinase substrate peptides on ready-to-use PepStar peptide microarrays

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PepStar™  Peptide Microarrays

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RepliTope™ Peptide Microarrays

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Contact us!

Please feel free to call or send us an email to peptide@jpt.com
+49-30-6392-7878
+1 978 631 4225 (US/Canada only)

JPT's Quality Assurance

All production is performed in JPT's head offices in Berlin, Germany according to ISO 9001:2008 standards

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