PepSpots™ Peptides on Cellulose

Peptide Arrays for Mapping Protein Interactions

JPT Peptide Technologies developed a well validated technology (Spot) to provide state-of-the-art B-cell epitope mapping in a reliable and speedy manner.
Mapped epitopes can be functionally characterized by rapid determination of key residues playing a definite role in the binding process. More than 100 scientific papers describing the application of this technology and the hundreds of successful mapping projects performed at JPT over the last decade distinguish the PepSpot™ technology as the method of choice for fast and reliable antibody epitope mapping.

Applications for PepSpots™ Peptides on Cellulose

• Antibody epitope mapping
• Functional characterization of mapped epitopes (e.g substitutional or truncation analyses):
  Which amino acids are critical for binding?
  Which amino acids are so called "key-residues”?
• Characterization of protein-protein contact sites, such as:
  • Receptor-ligand
  • Enzyme-substrate
  • Protein modules involved in signal transduction processes
  • Recognition sequences for heat shock proteins

Benefits of PepSpots™ Peptides on Cellulose

• Rapid, economical, and accurate synthesis using robotics
• Hydrophilic surface of cellulose membranes
• Minimization of unspecific hydrophobic interactions with target molecule
• Easy detection using standard ELISA protocols

Comparison of PepSpots™ Peptides on Celluloseand PepStar™ Peptide Microarrays

Take advantage of our comprehensive All-In-One Epitope Discovery Service

Send us an aliquot of your protein and the amino acid sequence of the binding partner and we will perform:

• Synthesis of PepSpot™ peptides
• Detection with your protein and data interpretation
• Functional characterization of mapped epitopes
• Complete and confidential documentation of results

Testimonials for PepSpots™ Peptides on Cellulose:

"My group at the University Clinics in Düsseldorf works on the investigation of protein misassembly, misfolding or misprocessing in brain diseases. Our research has an ongoing need for peptide related tools that allow systematic elucidation of such protein pathologies. JPT’s PepSpot arrays and JPT's ability to reproducibly assemble even challenging and modified peptides contributed to a noticeable degree to the success of our work. "
Prof. Dr. Carsten Korth, University of Düsseldorf, Germany

More testimonials under JPT Testimonials

Selected References for PepSpots™ Peptides on Cellulose:

Molecular Basis for the Unique Role of the AAA Chaperone ClpV in Type VI Protein Secretion
Pletrosluk et al., Journal of Biological Chemistry (2011)
Protein Kinase CK2 Regulates the Formation and Clearance of Aggresomes in Response to Stress
Watabe et al., J. Cell Sci. (2011)
HSP70 Natively and Specifically Associates with an N-terminal Dermcidin-derived Peptide That Contains an HLA-A*03 Antigenic Epitope
Stocki et al., J. Biol. Chem. (2011)
A Robust Protocol to Map Binding Sites of the 14-3-3 Interactome: Cdc25C Requires Phosphorylation of Both S216 and S263 to bind 14-3-3
Chan et al., Mol. Cell. Proteomics. (2011)
Biological Effects and Use of PrPSc- and PrP-Specific Antibodies Generated by Immunizing with Purified Full Length Native Mouse Prions
Petsch et al., J. Virol. (2011)
Cardiac Troponin T, a Sarcomeric AKAP, Tethers Protein Kinase A at the Myofilaments
Sumandea et al., J. Biol. Chem. (2011)
Inhibiting a-Synuclein Oligomerization by Stable Cell-Penetrating b-Synuclein Fragments Recovers Phenotype of Parkinson’s Disease Model Flies
Shaltiel-Karyo et al. PloS ONE (2010)
Identification of Potential HLA Class I and Class II Epitope Precursors Associated with Heat Shock Protein 70 (HSPA)
Stocki et al., Cell Stress Chaperones (2010)
A Monoclonal Antibody Against Mutated Nucleophosmin 1 for the Molecular Diagnosis of Acute Myeloid Leukemias
Gruszka et al. Blood (2010)
N-terminal Acetylation of Cellular Proteins Creates Specific Degradation Signals
Hwang HS et al, Science (2010)

More references under Publications/Literature