Applications for PepSpots™ Peptides on Cellulose

• Antibody epitope mapping
• Functional characterization of mapped epitopes (e.g substitutional or truncation analyses):
  Which amino acids are critical for binding?
  Which amino acids are so called "key-residues”?
• Characterization of protein-protein contact sites, such as:
  • Receptor-ligand
  • Enzyme-substrate
  • Protein modules involved in signal transduction processes
  • Recognition sequences for heat shock proteins

Benefits of PepSpots™ Peptides on Cellulose

• Rapid, economical, and accurate synthesis using robotics (SPOT Technology)
• Hydrophilic surface of cellulose membranes
• Minimization of unspecific hydrophobic interactions with target molecule
• Easy detection using standard ELISA protocols
• Take advantage of our comprehensive All-In-One Epitope Discovery Service

Testimonials for PepSpots™ Peptides on Cellulose:

"Our teams at the Vrije Universiteit Brussel deal with a wide variety of projects in different areas of applied biological sciences. For many of them we are in need of peptide related tools and services ranging from peptide libraries and highly purified peptides to peptide arrays. In our search for an integrated and reliable provider we came across JPT. Meanwhile, we are collaborating with JPT for many years and are very satisfied with the relationship which led to several well received publications. Especially, their unique array technology helped us to enhance our knowledge on different protein protein interactions such as Chaperone-substrate recognition."
Prof. Joost W.H. Schymkowitz & Prof. Frederic Rousseau, VIB Switch Laboratory, Vrije Universiteit Brussel, Belgium

More testimonials under JPT Testimonials

Selected References for PepSpots™ Peptides on Cellulose:

Molecular Basis for the Unique Role of the AAA Chaperone ClpV in Type VI Protein Secretion
Pletrosluk et al., Journal of Biological Chemistry (2011)
Protein Kinase CK2 Regulates the Formation and Clearance of Aggresomes in Response to Stress
Watabe et al., J. Cell Sci. (2011)
HSP70 Natively and Specifically Associates with an N-terminal Dermcidin-derived Peptide That Contains an HLA-A*03 Antigenic Epitope
Stocki et al., J. Biol. Chem. (2011)
A Robust Protocol to Map Binding Sites of the 14-3-3 Interactome: Cdc25C Requires Phosphorylation of Both S216 and S263 to bind 14-3-3
Chan et al., Mol. Cell. Proteomics. (2011)
Biological Effects and Use of PrPSc- and PrP-Specific Antibodies Generated by Immunizing with Purified Full Length Native Mouse Prions
Petsch et al., J. Virol. (2011)
Cardiac Troponin T, a Sarcomeric AKAP, Tethers Protein Kinase A at the Myofilaments
Sumandea et al., J. Biol. Chem. (2011)
Inhibiting a-Synuclein Oligomerization by Stable Cell-Penetrating b-Synuclein Fragments Recovers Phenotype of Parkinson’s Disease Model Flies
Shaltiel-Karyo et al. PloS ONE (2010)
Identification of Potential HLA Class I and Class II Epitope Precursors Associated with Heat Shock Protein 70 (HSPA)
Stocki et al., Cell Stress Chaperones (2010)
A Monoclonal Antibody Against Mutated Nucleophosmin 1 for the Molecular Diagnosis of Acute Myeloid Leukemias
Gruszka et al. Blood (2010)
N-terminal Acetylation of Cellular Proteins Creates Specific Degradation Signals
Hwang HS et al, Science (2010)

More references under Publications/Literature