Customized Products
Contract Research & Collaborations
Catalog Products

BioTides™ Biotinylated Peptides

Biotinylated peptides are used in a variety of biomedical screening systems requiring immobilization of at least one of the interaction partners onto streptavidine coated beads, membranes, glass slides or microtiter plates. By means of its proprietary SPOT synthesis technology, JPT Peptide Technologies provides access to large numbers of biotinylated peptides at unmatched prices and fastest delivery time.

Quote Request or contact our customer support via email

Applications for BioTides™ Biotinylated Peptides

  • Identification and optimization of kinase-, phosphatase-, acetyltransferase- and histone deacetylase-substrates via standard screening systems (AlphaScreen, FlashPlates, SPA-Beads, and many more
  • Mapping of protein/protein interaction sites (ELISA-like assays, precipitation of interacting proteins).
  • Production of peptide microarrays.
  • Loading of columns for affinity chromatography.

Bio Tides

Example: Human c-Jun derived BioTides and protein tyrosine kinase c-Abl (copy 1)

Used reagents and materials:

  • 384 well microtiter plate containing 160 different BioTides derived from human c-Jun protein
  • Abl-kinase (New England BioLabs, www.neb.com, item# P6050L)
  • Kinase buffer 50 mM Tris-HCl, 10 µM MgCl2 1 mM EGTA, 2 mM DTT, and 0.01% Brij 35 (pH 7.5, 25°C)
  • SAM-Biotin capture membrane (Promega, # V7861)
  • 384 pin replicator (Genetix, 384-pin QReps-PP, Product Code: X5050, printing volume approx: 0.2 µl)
PositionSEQ



Figure 1: Fluorescence image of SAM-Biotin capture membrane subsequent to antibody treatment

 

SAM-Membrane was incubated with FITC-labelled monoclonal anti-phosphotyrosine antibody PT-66 (Sigma, Order# F3145) (dilution 1:1000 in TBS) for 150 min followed by washings (5 times) with TBS-buffer. Fluorescence image was generated using FLA 3000 reader (Fuji, excitation at 473 nm, fluorescence filter 520 nm).





Figure 2: Chemiluminescence image of SAM-Biotin capture membrane subsequent to antibody treatment

AM-Membrane prepared using a diluted (1:100) assay solution was incubated with POD-labelled monoclonal anti-phosphotyrosine antibody PT-66 (Sigma, www.sigmaaldrich.com, Order# A5964) (dilution 1:1000 in TBS) for 240 min followed by washings (5 times) with TBS-buffer. Chemoluminescence image was generated using LumiImager (Roche Diagnostics) and BM chemiluminescence blotting substrate (Roche Diagnostics, # 1500694)






































A6DDALNASFLPSES
A7ALNASFLPSESGP
A8NASFLPSESGPYG
A9SFLPSESGPYGYS
A10LPSESGPYGYSNP
A11SESGPYGYSNPKI
A12SGPYGYSNPKILK
B1PYGYSNPKILKQS
B2GYSNPKILKQSMT
B3SNPKILKQSMTLN
B4PKILKQSMTLNLA
...
G5GSGSGGFSASLHS
G6GSGGFSASLHSEP
G7GGFSASLHSEPPV
G8FSASLHSEPPVYA
G9ASLHSEPPVYANL
G10LHSEPPVYANLSN
G11SEPPVYANLSNFN
G12PPVYANLSNFNPG
H1VYANLSNFNPGAL
H2ANLSNFNPGALSS
H3LSNFNPGALSSGG
H4NFNPGALSSGGGA
...
O1-O4ATP + Buffer
O5QSRRS-pT-QGVTL*
O6LRRRFS-pS-LHF*
O7NDEN-pY-HPRESS*
O8Kinase
O9Buffer
O10QSRRS-pT-QGVTL*
O11LRRRFS-pS-LHF*
O12NDEN-pY-HPRESS*
P1Kinase + ATP
P2Kinase + ATP
P3Kinase + ATP
P4Kinase + ATP
P5QSRRS-pT-QGVTL*
P6LRRRFS-pS-LHF*
P7NDEN-pY-HPRESS*
P8Kinase
P9Buffer
P10QSRRS-pT-QGVTL*
P11LRRRFS-pS-LHF*
P12NDEN-pY-HPRESS*
pS/pT/pY positive controls* pS=phospho-Ser; pT=phospho-Thr; pY=phospho-Tyr
negative controls
G8-H1known phosphorylation-site: Barila D, Mangano R, Gonfloni S, Kretzschmar J, Moro M, Bohmann D, Superti-Furga G.; A nuclear tyrosine phosphorylation circuit: c-Jun as an activator and substrate of c-Abl and JNK; EMBO J. 2000 Jan 17;19(2):273-81

Testimonials for BioTides™ Biotinylated Peptides:

"Our group at OHSU has purchased more than 50 000 peptides over the past few years from JPT to support our proteome wide TB epitope discovery program.  We are impressed by the turnaround times and highly competitive pricing structure for such large numbers of peptides and are very satisfied with the responsive and professional service."
Gwendolyne Swarbrick, PhD, Oregon Health & Science University, Portland, USA

More testimonials under JPT Testimonials

© JPT Peptide Technologies GmbH
print