Protease Substrate Set

JPT Peptide Technologies’ Protease Substrate Sets are ready-to-use tools to study protease activity and substrate specificity. The Protease Substrate Sets comprise of internally quenched (Dabcyl/EDANS) peptides derived from proteolytic cleavage sites (from P4 to P4’position).The purified and lyophilized peptides come in a 384-well microtiter plate (75 pmol/well). Subsequent to incubation with target protease the evolving fluorescence can be measured using standard fluorescence plate reader systems.

• Protease Substrate Set (360 peptides, #PrSS-360-75, sequences and microtiter plate layout)
• Caspase Substrate Set (4 x 88 peptides, #PrSS-C88-75, sequences and microtiter plate layout)

Benefits of Protease Substrate Sets

• Screen 360 protease substrates efficiently (2520 potential cleavage sites)
• Screen 88 caspase substrates derived from human cleavage sites efficiently
• Save time for HTS-Assay development
• Learn more about the biology of your protease
• Identification of potential in vivo substrates
• Determine kinetic constants

Applications for Protease Substrate Sets

• Identification of protease substrates and cleavage sites
• Profiling of protease substrate specificities
• Determination of contaminating proteases
• Examination of protease inhibitors

Related products and services

ProteaseSpots™
Customized fluorescent peptides bound to cellulose discs in 96-well microtiter plates for protease profiling.

Protease Peptide Microarrays
Compilation of protease substrate peptides on ready-to-use PepStar™ peptide microarrays.

Protease Profiling Service
Profiling service for protease using high-content PepStar™ peptide microarrays.