Kinase Profiling Service

JPT Peptide Technologies GmbH has compiled a unique database of human phosphorylation sites. Peptides representing these phosphorylation sites are displayed on PepStar™ peptide microarrays in triplicates ensuring data quality.
In our Enzyme Profiling Service, we will profile your kinase at our facility within one week of receipt of the enzyme. You will receive the comprehensive data evaluation including the scanned microarray images after incubation with the target enzyme, statistical analyses of the results, and disclosure of the top 20 substrate sequence information.

The following high density peptide microarrays are available in our Enzyme Profiling Service exclusively:

Please inquire for customized peptide microarrays tailor-made to your order.

Annotated Phosphosites-K

The microarray containing 2 304 annotated human phosphorylation sites allows both kinase substrate identification and consensus sequence determination. Each identified in vitro substrate peptide corresponds to a human protein, which represents a potential in vivo target of the studied kinase.

Activation Loop Scan

The Activation Loop Scan peptide microarray contains 1 600 sequences from activation loops of all human kinases. Identified hits on this peptide microarray correspond to potential downstream kinases possibly activated by the studied enzyme.

Membrane Protein Scan

Our peptide microarrays comprise a total of 11 098 peptides containing 21 349 potential phosphorylation sites from cytoplasmic domains of human membrane proteins. Screening of the complete or partial library results in the convenient identification of target receptor sites and auto-phosphorylation sites of receptor tyrosine kinases.

Priming Phosphorylation Detector

Priming phosphorylation events can be identified in two ways using our PepStar™ peptide technology.

(1) Screening with target kinase of a PhosphoSite™ microarray, which was already phosphorylated by another kinase.
(2) Screening of peptide microarrays of synthetically phosphorylated peptides containing additional phosphosites.

Priming phosphorylation observed in vitro will lead the discovery of in vivo events from which conclusions can be drawn on signaling pathways.