Kinase Peptide Microarrays

JPT Peptide Technologies GmbH has compiled a unique database of human phosphorylation sites. Peptides representing these phosphorylation sites are displayed on PepStar™ peptide microarrays in triplicates ensuring data quality. Peptide derivatives are displayed in an arrangement similar to 384-well microtiter plates on each Kinase Peptide Microarray to simplify data evaluation.

The following peptide microarrays are available:

Please inquire for customized peptide microarrays tailor-made to your order.

Related products and services

Single Kinase Substrate Peptides
The substrates represent 13meric peptides derived from phosphorylation sites of human proteins. Please select from
the list of nearly 800 peptides currently available.

Phosphorylation Site Detector
Compilation of kinase substrate peptides on ready-to-use
PepStar™ peptide microarrays.

Kinase Substrate Sets
Compilation of kinase substrate peptides in ready-to-use
microtiter plates.

Kinase Profiling Service
Profiling service for kinases using high-content
PepStar™ peptide microarrays.

Annotated Phosphosites-K

The microarrays containing 720 peptides derived from human phosphosites sites allow both kinase substrate identification and consensus sequence determination. They come as two microarrays each displaying 360 of the Kinase Substrate Set peptides in three identical subarrays resulting in 1 080 deposited peptides. Each identified in vitro substrate peptide corresponds to a known protein, which represents a potential in vivo target of the studied kinase.

Download microarray layout and sequence information (PDF-File).

Random Library Tyrosine-K

This peptide microarray displays 1 536 peptides containing one tyrosine residue in the center position. Screening of the complete or partial library results in the identification of peptidic substrates of the target tyrosine kinase.

Download microarray layout and sequence information (PDF-File).

Random Library Threonine-K

This peptide microarray displays 1 536 peptides containing one threonine residue in the center position. Screening of the complete or partial library results in the identification of peptidic substrates of the target serine/threonine kinase.

Download microarray layout and sequence information (PDF-File).

Random Library Serine-K

This peptide microarray displays 1 536 peptides containing one serine residue in the center position. Screening of the complete or partial library results in the identification of peptidic substrates of the target serine/threonine kinase.

Download microarray layout and sequence information (PDF-File).

References:

• Proteomic Analysis of in Vivo Phosphorylated Synaptic Proteins, Collins et al., J. Biol. Chem., Vol. 280:7 (2005), p. 5972-5982 (abstract)

• Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening, Mah et al., Biochemistry, Vol. 6:22 (2005) (abstract)

• Kinomics: methods for deciphering the kinome, Johnson and Hunter, Nature Methods, Vol. 2 (2005), p. 17–25 (abstract)

• Profiling of generic anti-phosphopeptide antibodies and kinases with peptide microarrays using radioactive and fluorescence-based assays, Panse et al., Molecular Diversity, Vol. 8 (2004), p. 291–299 (abstract)

• High-Content Peptide Microarrays for Deciphering Kinase Specificity and Biology, Schutkowski et al., Angew. Chem. Int. Ed., Volume 43, Issue 20 (2004), p. 2671 - 2674 (abstract)

• Target Specificity Analysis of the Abl Kinase using Peptide Microarray Data, Rychlewski et al., J. Mol. Biol. Vol. 336 (2004), p. 307–311 (abstract)