Kinase Specificity Profiling
JPT provides access to identification of substrates specific within a panel of given kinases. Substitutional analyses of either known substrates or substrates identified using JPT´s Kinase Peptide Microarrays will be immobilized onto glass slides. Resulting peptide microarray will be screened using your kinases of interest in the presence of radioisotopically labelled ATP followed by phosphorimaging. JPT will perform all experiments and will deliver a data report including experimental details, phosphorimages and analysis of results yielding specific substrates. Resulting substrates could be optimized in subsequent substitutional analysis experiment including non-proteinogenic amino acid residues.
Benefits of Kinase Specificity Profiling
- Information about specific substrates available within days
- Production of customized substitutional analyses on glass slides within less than 3 weeks
- Optimizes surfaces and built-in quality controls
- Small amount of sample needed (200 µL per peptide microarray)
Applications of Kinase Specificity Profiling
- Identify key residues for effective kinase/kinase substrate interaction enabling prediction of kinase substrates
- Find substrates specific for your target kinase
- Optimize your known kinase substrate regarding stability, selectivity and catalytic constants
- Learn more about your kinase by chemical introduction of other posttranslational modifications into sequences surrounding the phosphorylation site of your substrate
Related products and services
Phosphorylation Site Detector
Ready-to-use PepStar™ peptide microarrays displaying overlapping peptide scans through known kinase substrate proteins
(full service available)
Kinase Peptide Microarrays
Peptide microarrays containing either annotated human phosphorylation site derived peptides or randomly generated peptide for identification of kinase substrates and profiling of kinase substrate specificities.
Kinase Substrate Identification
Kinase substrate identification service using JPT's peptide collections and microarrays
Phosphorylation Site Detection
Economical detection of phosphosites in kinase substrate proteins within two weeks
Selected References:
- Molecular Basis for the Substrate Specificity of NIMA-Related Kinase-6 (NEK6). Evidence that NEK6 Does not Phosphorylate the Hydrophobic Motif of Ribosomal S6 Protein Kinase and Serum- and Glucocorticoid-Induced Protein Kinase In Vivo.
Lizcano et al., J Biol Chem (2002) (abstract) - High-Content Peptide Microarrays for Deciphering Kinase Specificity and Biology
Schutkowski et al., Angew. Chem. (2004) (abstract) - Target Specificity Analysis of the Abl Kinase using Peptide Microarray Data
Rychlewski et al., J. Mol. Biol. (2004) (abstract) - Substrate Specificity Analysis of Protein Kinase Complex Dbf2-Mob1 by Peptide Library and Proteome Array Screening
Mah et al., Biochemistry (2005) (abstract)
More references under JPT Publications/Literature
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