Protease Substrate Sets
JPT Peptide Technologies’ Protease Substrate Sets are ready-to-use tools to study protease activity and substrate specificity. The Protease Substrate Sets are comprised of internally quenched (Dabcyl/EDANS) peptides derived from proteolytic cleavage sites (from P4 to P4’position). The purified and lyophilized peptides come in a 384-well microtiter plate (75 pmol/well). Subsequent to incubation with target protease, the evolving fluorescence can be measured using standard fluorescence plate reader systems.
Two sets are available:
- Protease Substrate Set (360 peptides, sequences and microtiter plate layout)
- Caspase Substrate Set (4 x 88 peptides, sequences and microtiter plate layout)
Benefits of Protease Substrate Sets
- Screen 360 protease substrates efficiently (2520 potential cleavage sites)
- Screen 88 caspase substrates derived from human cleavage sites efficiently
- Save time for HTS-Assay development
- Learn more about the biology of your protease
- Identification of potential in vivo substrates
- Determine kinetic constants
Applications for Protease Substrate Sets
- Identification of protease substrates and cleavage sites
- Profiling of protease substrate specificities
- Determination of contaminating proteases
- Examination of protease inhibitors
Related products and services
ProteaseSpots™
Customized fluorescent peptides bound to cellulose discs in 96-well microtiter plates for protease profiling.
Protease Substrate Identification
Profiling service for protease using high-content PepStar™ peptide microarrays.
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