Products

Phosphorylation Site Detector


JPT Peptide Technologies offers an efficient way to detect potential phosphorylation sites in kinase substrate proteins. Each Phosphorylation Site Detector represents a single peptide microarray on a glass slide containing an overlapping peptide scan through known proteins (please refer to table below). Subsequent to incubation with target kinase in the presence of radioactive ATP, incorporated phosphate can be detected by autoradiography or phosphorimaging. Alternatively, phospho-specific antibody-based fluorescence readout is applicable for phosphopeptide detection.

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Benefits - Phosphorylation Site Detector microarrays

  • Save time and material to determine phosphosites in proteins
  • Screen several hundred peptides economically
  • Learn more about your kinase/kinase target protein

Applications - Phosphorylation Site Detector microarrays

  • Identification of kinase substrate peptides
  • Detection of potential phosphosites in substrate proteins
  • Determination of auto-phosphorylation sites
  • Identification of upstream kinases for your target protein
  • Elucidation of signal transduction pathways

Available Phosphorylation Site Detector microarrays


Product-Code Protein Organism ID-Code
number		 Primary
accession
number		 Database Peptides
per
subarray		 Scan
type*
PD-001 14-3-3 zeta homo sapiens NP_003397 NP_003397 Swiss-Prot 79 13/10
PD-002 AKT1 homo sapiens NP_005154 NP_005154 NCBI 157 13/10
PD-003 BCL 2 homo sapiens NP_004313 NP_004313 NCBI 79 13/11
PD-004 Beta-casein homo sapiens P05814 P05814 Swiss-Prot 67 13/10
PD-005 B-Myb homo sapiens NP_002457 NP_002457 NCBI 345 13/11
PD-006 CD5 antigene homo sapiens NP_055022 NP_055022 NCBI 41 13/11
PD-007 Cdc25C homo sapiens NP_001781 NP_001781 NCBI 155 13/10
PD-008 CHK2 homo sapiens NP_009125 NP_009125 NCBI 178 13/10
PD-009 c-jun homo sapiens NP_002219 NP_002219 NCBI 160 13/11
PD-010 C-Nap1(CEP2) homo sapiens CEP2_HUMAN Q9BV73 Swiss-Prot 194
 13/10
PD-011 Connexin 43 homo sapiens NP_000156 NP_000156 NCBI 94 13/9
PD-012 crystallin B homo sapiens NP_001876 NP_001876 NCBI 82 13/11
PD-013 Cyp11 homo sapiens PPIF_HUMAN P30405 Swiss-Prot 195 13/12
PD-014 Cyp18.1 homo sapiens PPIL3_HUMAN Q9H2H8 TrEMBL 149 13/12
OD-015 Cyp18.2 homo sapiens Q9Y536_HUMAN Q9Y536 TrEMBL 152 13/12
PD-016 Cyp18.6 homo sapiens PPIL3_HUMAN Q9BXZ1 TrEMBL 153 13/12
PD-017 Cyp19.2 homo sapiens PPIH_HUMAN O43447 Swiss-Prot 165 13/12
PD-018 Cyp23 homo sapiens PPIB_HUMAN P23284 Swiss-Prot 196 13/12
PD-019 CYPA homo sapiens PPIA_HUMAN P62937 Swiss-Prot 77 13/11
PD-020 E2F homo sapiens NP_005216 NP_005216 NCBI 143 13/10
PD-021 EpoR homo sapiens NP_000112 NP_000112 NCBI 75 13/10
PD-022 FKBB12.6 homo sapiens FKBB_HUMAN P68106 Swiss-Prot 48 13/11
PD-023 FKBP12 homo sapiens FKB1_HUMAN P62942 Swiss-Prot 48 13/11
PD-024 FKBP13 homo sapiens FKB2_HUMAN P26885 Swiss-Prot 66 13/11
PD-025 FKBP135 homo sapiens Q9Y4D0 Q9Y4D0 TrEMBL 205 13/7
PD-026 FKBP19 homo sapiens FK11_HUMAN Q9NYL4 Swiss-Prot 95 13/11
PD-027 FKBP22 homo sapiens FK14_HUMAN Q9NWM8 Swiss-Prot 100 13/11
PD-028 FKBP23 homo sapiens FKB7_HUMAN Q9Y680 Swiss-Prot 124 13/11
PD-029 FKBP25 homo sapiens FKB3_HUMAN Q00688 Swiss-Prot 107 13/11
PD-030 FKBP36 homo sapiens FKB6_HUMAN O75344 Swiss-Prot 106 13/10
PD-031 FKBP37 homo sapiens AIP_HUMAN O00170 Swiss-Prot 107 13/10
PD-032 FKBP38 homo sapiens FKB8_HUMAN Q14318 Swiss-Prot 115 13/10
PD-033 FKBP44 homo sapiens AIPL_HUMAN Q9NZN9 Swiss-Prot 125 13/10
PD-034 FKBP51 homo sapiens FKB5_HUMAN Q13451 Swiss-Prot 149 13/10
PD-035 FKBP52 homo sapiens FKB4_HUMAN Q02790 Swiss-Prot 150 13/10
PD-036 GAB1 homo sapiens NP_002030 NP_002030 NCBI 342 13/11
PD-037 Histone H1 bos taurus H11_BOVIN P02253 Swiss-Prot 47 13/11
PD-038 LKB1 homo sapiens NP_000446 NP_000446 NCBI 141 13/10
PD-039 MBP bos taurus P02687   Swiss-Prot 141 13/11
PD-040 MDM2 homo sapiens NP_002383 NP_002383 NCBI 161 13/10
PD-041 MeCP2 homo sapiens NP_004983 NP_004983 NCBI 159 13/10
PD-042 NEK 2 homo sapiens NEK2_HUMAN P51955 Swiss-Prot 217 13/11
PD-043 NFATP homo sapiens NP_775114 NP_775114 NCBI 305 13/10
PD-044 Nucleophosmin 1 homo sapiens NP_002511 NP_002511 NCBI 95 13/10
PD-045 p53 homo sapiens NP_000537 NP_000537 NCBI 96 13/9
PD-046 PAK1 homo sapiens AAC24716 AAC24716 NCBI 181 13/10
PD-047 Parvulin14 homo sapiens PIN4_HUMAN Q9Y237 Swiss-Prot 60
 13/11
PD-048 PDK1 homo sapiens NP_002604 NP_002604 NCBI 182 13/10
PD-049 Phas-I homo sapiens NP_004086 NP_004086 NCBI 54 13/11
PD-050 Pin 1 homo sapiens PIN1_HUMAN Q13526 Swiss-Prot 76 13/11
PD-051 Pin 1 digitalis lanata PIN1_DIGLA Q9LEK8 Swiss-Prot 54
 13/11
PD-052 PTEN homo sapiens NP_000305 NP_000305 NCBI 196 13/11
PD-053 PTMA homo sapiens NP_002814 NP_002814 NCBI 50 13/11
PD-054 PTPN1 homo sapiens NP_002818 NP_002818 NCBI 142 13/10
PD-055 RAD9 homo sapiens NP_004575 NP_004575 NCBI 96 13/9
PD-056 RAF1 homo sapiens NP_002871 NP_002871 NCBI 319 13/11
PD-057 RBL2 homo sapiens NP_005602 NP_005602 NCBI 377 13/10
PD-058 S6 protein homo sapiens NP_001001 NP_001001 NCBI 80 13/10
PD-059 Tau homo sapiens TAU_HUMAN P10636 Swiss-Prot 373 13/11
PD-060 Tie 2 homo sapiens TIE2_HUMAN Q02763 Swiss-Prot 172 13/11

* The first number X indicates the total number of amino acids of the peptide-sequence, whereas the second number Y indicates the number of overlapping amino acids.


Figure:
For quality control, three identical subarrays (including controls) are printed to each single microarray. All peptides are chemoselectively immobilized via their N-terminus. The hydrophilic linker moiety 1-amino-4,7,10-trioxa-13-tridecanamine succinimic acid is used as a spacer between the peptides and the glass surface.


Figure:
The full-length control proteins are covalently attached by non-selective immobilisation chemistry using the amino-function of the lysine-side chains. We can not guarantee that the proteins are natively folded.

  Protein Company Cat#
VIII Tau-Protein Sigma T-9392
VII β-Casein Sigma C-6905
VI α-Casein Sigma C-7891
V MBP Biomol- Upstate 13-104
IV Histon 4 Biomol- Upstate 14-412
III Histon 3 Biomol- Upstate 14-411
II Histon 2A Biomol- Upstate 14-409
I Histon 1 Biomol- Upstate 14-155

Selected References:

  • Proteomic Analysis of in Vivo Phosphorylated Synaptic Proteins
    Collins et al., J. Biol. Chem., Vol. 280:7 (2005), pp. 5972-5982 (abstract)

    More references under JPT Publications/Literature

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