Kinase Substrate Identification
JPT provides highly comprehensive service to find substrates for your kinases using both peptides immobilized to glass surfaces (Kinase Peptides Microarrays) and soluble peptides in 384-well microtiter plates (Kinase Substrate Sets).
Discuss your needs with our scientists and simply send us your kinase sample. JPT will perform all experiments and will deliver a data report including experimental details and statistical analysis of results yielding consensus sequence motifs.
Benefits of Kinase Substrate Identification
- Select from more than 90 000 human peptides available on peptide microarrays
- Receive information about your kinase substrates and consensus sequence within two weeks
- Get information about potential downstream targets of your kinase
- Identify kinase contaminations in your sample
- Minimal sample volume needed (200 µL assay volume per peptide microarray)
Related products and services
Single Kinase Substrate Peptides
The substrates represent 13meric peptides derived from phosphorylation sites of human proteins.
Please select from the list of nearly 800 peptides currently available.
Kinase Peptide Microarrays
Peptide microarrays containing either annotated human phosphorylation site derived peptides or randomly generated peptide for identification of kinase substrates and profiling of kinase substrate specificities.
Kinase Substrate Sets
Compilation of kinase substrate peptides in ready-to-use microtiter plates.
Phosphatase Substrate Identification
Phosphatase substrate identification service using JPT's peptide collections and microarrays
Protease Substrate Identification
Protease substrate identification service using JPT's peptide collections and microarrays
Selected References:
- Substrate Specificity Analysis of Protein Kinase Complex Dbf2-Mob1 by Peptide Library and Proteome Array Screening
Mah et al., Biochemistry (2005) (abstract) - Profiling of Generic Anti-Phosphopeptide Antibodies and Kinases with Peptide Microarrays Using Radioactive and Fluorescence-Based Assays
Panse et al., Molecular Diversity (2004) (abstract) - High-Content Peptide Microarrays for Deciphering Kinase Specificity and Biology
Schutkowski et al., Angew. Chem. Int. Ed. (2004) (abstract) - Target Specificity Analysis of the Abl Kinase using Peptide Microarray Data
Rychlewski et al., J. Mol. Biol. (2004) (abstract)
More references under JPT Publications/Literature
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