Annotated Phosphosites-K

The microarrays containing 720 peptides derived from human phosphosites sites allow both kinase substrate identification and consensus sequence determination. They come as two microarrays each displaying 360 of the Kinase Substrate Set peptides in three identical subarrays resulting in 1 080 deposited peptides. Each identified in vitro substrate peptide corresponds to a known protein, which represents a potential in vivo target of the studied kinase (microarray layout and sequence information).

Random Library Tyrosine-K

These four peptide microarrays each displaying 384 peptides comprise a total of 1 536 peptides containing one tyrosine residue in the center position. Screening of the complete or partial library results in the identification of peptidic substrates of the target tyrosine kinase (microarray layout and sequence information).

Random Library Threonine-K

These four peptide microarrays each displaying 384 peptides comprise a total of 1 536 peptides containing one threonine residue in the center position. Screening of the complete or partial library results in the identification of peptidic substrates of the target serine/threonine kinase (microarray layout and sequence information).

Random Library Serine-K

These four peptide microarrays each displaying 384 peptides comprise a total of 1 536 peptides containing one serine residue in the center position. Screening of the complete or partial library results in the identification of peptidic substrates of the target serine/threonine kinase (microarray layout and sequence information).

Related products and services

Single Kinase Substrate Peptides
The substrates represent 13meric peptides derived from phosphorylation sites of human proteins.
Please select from the list of nearly 800 peptides currently available.

Phosphorylation Site Detector
Ready-to-use PepStar™ peptide microarrays displaying overlapping peptide scans through known kinase substrate proteins
(full service available).

Kinase Substrate Sets
Compilation of kinase substrate peptides in ready-to-use microtiter plates.

Kinase Substrate identification
Profiling service for kinases using high-content PepStar™ peptide microarrays.

Testimonials

"We have successfully applied JPT's peptide microarrays as a screening tool for potential kinases transfering phosphate to phosphorylation sites identified by mass spectrometry. The triplicate alignment as well as the thorough selection of controls enabled generation of high quality data confirming our in vivo data generated by means of affinity chromatographic procedures."

Dr. Marcelo P. Coba (The Wellcome Trust Sanger Institute, Cambridge, UK)

Selected References:

• Proteomic Analysis of in Vivo Phosphorylated Synaptic Proteins
  Collins et al., J. Biol. Chem. (2005), (abstract)
• Substrate Specificity Analysis of Protein Kinase Complex Dbf2-Mob1 by Peptide Library and Proteome Array Screening
  Mah et al., Biochemistry (2005) (abstract)
• Kinomics: Methods for Deciphering the Kinome
  Johnson and Hunter, Nature Methods (2005) (abstract)
• Profiling of Generic Anti-Phosphopeptide Antibodies and Kinases with Peptide Microarrays Using Radioactive and Fluorescence-Based Assays
  Panse et al., Molecular Diversity (2004) (abstract)
• High-Content Peptide Microarrays for Deciphering Kinase Specificity and Biology
  Schutkowski et al., Angew. Chem. (2004) (abstract)
• Target Specificity Analysis of the Abl Kinase using Peptide Microarray Data
  Rychlewski et al., J. Mol. Biol. (2004) (abstract)
  
  More references under JPT Publications/Literature