PepStar™
JPT Peptide Technologies applies its unique PepStar™ peptide microarray platform to generate customized peptide microarrays on glass slides for biomarker discovery, immuno monitoring and detection and validation of protein interactions. In addition, PepStar™ technology also enables characterization of enzyme target families such as kinases, phosphatases and proteases as well as methyl, glycosyl, acetyl transferases and lysine deacetylases.
Peptides are immobilized on glass slides via a flexible linker. Chemoselective coupling generates microarrays of directed and covalently attached peptides. Multiple identical copies of peptide microarrays can be prepared at unmatched prices and turnaround times. Incubation can be performed manually or automated and read-out is performed via fluorescence. This results in low backgrounds and high sensitivity.
Applications:
- Mapping of immunodominant regions in antigens
- Identification of substrates for orphan enzymes
- Optimization of known enzyme substrates
- Elucidation of signal transduction pathways
- Reliable detection of contaminating enzyme activities
Benefits:
- Get hundreds of identical microarray copies from one peptide synthesis
- Peptides are free of truncated sequences
- Small amounts of your precious samples are needed for incubation
- Low background on glass surface
- Read-out via fluorescence
- Save time for HTS assay setup
- Screen thousands of potential peptide substrates economically
- Learn more about the biology of your enzyme
Selected References:
- Pattern Recognition in Pulmonary Tuberculosis Defined by High Content Peptide Microarray Chip Analysis Representing 61 Proteins from M. tuberculosis.
Gatseitsiwe et al., PloS One (2008) (abstract) - Validation of Peptide Epitope Microarray Experiments and Extraction of Quality Data
Nahtman et al., J. Immunol. (2007) (abstract) - Prion Recognition Elements Govern Nucleation, Strain Specificity and Species Barriers
Tessier & Lindquist, Nature (2007) (abstract) - High-Content Peptide Microarrays for Deciphering Kinase Specificity and Biology
Schutkowski et al., Angew. Chem. Int. Ed. (2004) (abstract) - Profiling of Generic Anti-Phosphopeptide Antibodies and Kinases with Peptide Microarrays Using Radioactive and Fluorescence-Based Assays
Panse et al., Molecular Diversity (2004) (abstract) - Peptide Arrays: from Macro to Micro
Reimer et al., Curr. Opin. Biotechnol. (2002) (abstract)
More references under JPT Publications/Literature
Testimonials
"One focus of our group is to decipher the nature of immune responses by identification of biomarkers and indicators of immune protection. With the support of JPT's high content peptide microarray platform, we created a peptide chip which contains 22 000 individual peptides. This enabled the visualization of the B-cell "signature" in individuals with TB-infection vs. non- infected individuals. In our hands, JPT's peptide microarrays turned out to be very robust tools to identify novel peptide based biomarkers in the context of novel diagnostics and vaccine target identification."
Prof. Markus Maeurer, Karolinska Institute, Solna, Sweden
Related products and services
Epitope Discovery Service
Take advantage of our comprehensive Full Service (incl. incubation, analyses and detailed report)
RepliTope™
Peptide chips for mapping immunodominant regions in antigens
Phosphorylation Site Detector and Kinase Peptide Microarrays
Compilation of kinase substrate peptides on ready-to-use PepStar peptide microarrays
Kinase Substrate Identification
Profiling service for kinases using high content PepStar peptide microarrays
Phosphatase Peptide Microarrays
Compilation of phosphatase substrate peptides on ready-to-use PepStar peptide microarrays
Protease Peptide Microarrays
Compilation of protease substrate peptides on ready-to-use PepStar peptide microarrays
Protease Substrate Identification
Profiling service for proteases using high content PepStar peptide microarrays
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