Peptide Arrays for Mapping Protein Interactions

JPT Peptide Technologies developed a well validated technology (Spot) to provide state-of-the-art B-cell epitope mapping in a reliable and speedy manner.
Mapped epitopes can be functionally characterized by rapid determination of key residues playing a definite role in the binding process.
More than 100 scientific papers describing the application of this technology and the hundreds of successful mapping projects performed at JPT over the last decade distinguish the PepSpot™ technology as the method of choice for fast and reliable antibody epitope mapping.

Applications for PepSpots™

• Antibody epitope mapping
• Functional characterization of mapped epitopes (e.g substitutional or truncation analyses):
  Which amino acids are critical for binding?
  Which amino acids are so called "key-residues”?
• Characterization of protein-protein contact sites, such as:
  
  • Receptor-ligand
  • Enzyme-substrate
  • Protein modules involved in signal transduction processes
  • Recognition sequences for heat shock proteins

Benefits of PepSpots™ peptide arrays

• Rapid, economical, and accurate synthesis using robotics
• Hydrophilic surface of cellulose membranes
• Minimization of unspecific hydrophobic interactions with target molecule
• Easy detection using standard ELISA protocols

Comparison of PepSpots™ and PepStar™

Take advantage of our comprehensive All-In-One Epitope Discovery Service

Send us an aliquot of your protein and the amino acid sequence of the binding partner and we will perform:

• Synthesis of PepSpot™ peptides
• Detection with your protein and data interpretation
• Functional characterization of mapped epitopes
• Complete and confidential documentation of results

Selected References:

• Peptide Arrays on Cellulose Support: SPOT Synthesis, a Time and Cost Efficient Method for Synthesis of Large Numbers of Peptides in a Parallel and Addressable Fashion
  Hilpert et al., Nature Protocols (2007) (abstract)
• Structure-Based Epitope and PEGylation Sites Mapping of Phenylalanine Ammonia-Lyase for Enzyme Substitution Treatment of Phenylketonuria
  Gamez et al., Mol. Genet. Metab., (2007) (abstract)
• Analysis of Antibody Response in Humans to the Type A OspC Loop 5 Domain and Assessment of the Potential Utility of the Loop 5 Epitope in Lyme Disease Vaccine Development
  Buckles et al., Clin. Vaccine Immunol. (2006) (abstract)
• Identification of Distinct Antibody Epitopes and Mimotopes from a Peptide Array of 5520 Randomly Generated Sequences
  Reineke et al., J. Immun. Methods (2002) (abstract)
• Peptide Arrays: from Macro to Micro
  Reimer et al., Curr. Opin. Biotechnol. (2002) (abstract)
• Applications of Peptide Arrays Prepared by the SPOT-Technology
  Reineke et al., Curr. Opin. Biotechnol. (2001) (abstract)
• Coherent Membrane Supports for Parallel Microsynthesis and Screening of Bioactive Peptides
  Wenschuh et al., Biopolymers (2000) (abstract)
• Antigen Sequence and Library-Based Mapping of Linear and Discontinuous Protein-Protein-Interaction Sites by Spot Synthesis
  Reineke et al., Curr. Top. Microbiol. Immunol. (1999) (abstract)
• Molecular Basis for the Binding Promiscuity of an Anti-p24 (HIV-1) Monoclonal Antibody
  Kramer et al., Cell (1997) (abstract)
  
  More references under Publications/Literature

Testimonials:

"The main focus of my research group at the Charité in Berlin is the development of novel immunotherapeutic approaches against cancer and infectious diseases. For reliable monitoring of tumor and virus specific T-cell responses we have a permanent need for peptides and peptide pools that are produced in a regulated environment for application in a clinical environment. JPT has been a long term and dedicated partner in this regard which continuously works on improving it's peptide based services."
Prof. Dr. Carmen Scheibenbogen, Charité Berlin, Germany

"My group at the University Clinics in Düsseldorf works on the investigation of protein misassembly, misfolding or misprocessing in brain diseases. Our research has an ongoing need for peptide related tools that allow systematic elucidation of such protein pathologies. JPT’s PepSpot arrays and JPT's ability to reproducibly assemble even challenging and modified peptides contributed to a noticeable degree to the success of our work. "
Prof. Dr. Carsten Korth, University of Düsseldorf, Germany